A simple device to demonstrate the principles of fluorometry
A simple device to demonstrate the principles of fluorometry
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DOI：
10.1021/ed076p1265
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发表时间：
1999-09-01
影响因子：
3
通讯作者：
Gatti, CA
中科院分区：
文献类型：
--
作者：
Delorenzi, NJ;Araujo, C;Gatti, CA
用容易获得的低成本部件建造了一个简单的装置来演示荧光法的原理。这些组件是一个微型紫外线荧光灯、一个塑料电池支架、一个硼硅酸盐玻璃管和一个光敏电阻(LDR)。用万用表与计算机接口进行数据采集和处理，测量LDR电阻。建立了硫酸氢奎宁(QBS)溶液的荧光与LDR电阻之间的校正曲线。用Stern-Volmer方法研究了氯化钠对QBS的荧光猝灭作用。该装置的设计允许直接观察荧光现象的下列特征：(I)激发光和发射光之间的波长漂移，(Ii)发射强度随着荧光团浓度的增加而增加，以及(Iii)高浓度QBS溶液的内滤波效应。
A simple device to demonstrate the principles of fluorimetry was constructed using low-cost components that are readily obtained. These components were a mini ultraviolet fluorescent lamp, a plastic cell holder, borosilicate glass tubes, and a photoresistor (LDR). The LDR resistance was measured by a multimeter interfaced to a computer for data acquisition and processing. A calibration curve was constructed to relate the fluorescence of quinine bisulfate (QBS) solutions with the LDR resistance. QBS fluorescence quenching by NaCl was studied by the Stern-Volmer approach. The apparatus was designed to permit the direct observation of the following features of the fluorescence phenomenon: (i) the wavelength shift between excitation and emission light, (ii) the increase of emission intensity with increasing fluorophore concentration, and (iii) the inner filter effect for highly concentrated QBS solutions.