Gene Models, Expression Repertoire, and Immune Response of Plasmodium vivax Reticulocyte Binding Proteins.
Gene Models, Expression Repertoire, and Immune Response of Plasmodium vivax Reticulocyte Binding Proteins.
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基因模型，表达曲目和疟原虫疟原虫网状细胞结合蛋白的免疫反应。
DOI：
10.1128/iai.01117-15
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发表时间：
2015-12-28
影响因子：
3.1
通讯作者：
Nguitragool W
中科院分区：
文献类型：
--
作者：
Hietanen J;Chim-Ong A;Chiramanewong T;Gruszczyk J;Roobsoong W;Tham WH;Sattabongkot J;Nguitragool W
间日疟原虫网织红细胞结合蛋白（PvRBP）家族的成员被认为介导间日疟原虫对网织红细胞的特异性侵袭。在这项研究中，我们进行了编码该蛋白质家族成员的基因的分子特征。通过cDNA测序，我们构建了全长基因模型，并验证了蛋白质编码基因和假基因。我们还使用定量PCR来测量它们在临床间日疟原虫分离株中的体内转录丰度。与编码恶性疟原虫的相关侵袭配体的基因一样，Pvrbp表达水平在不同的寄生虫分离株之间变化很大。通过抗体测量，我们发现宿主免疫压力可能是家族成员之一PvRBP2c明显高多样性背后的驱动力。轻度但显着的负相关性之间的寄生虫血症和PvRBP2b抗体水平，表明抗体的蛋白质可能会干扰入侵。
Members of the Plasmodium vivax reticulocyte binding protein (PvRBP) family are believed to mediate specific invasion of reticulocytes by P. vivax. In this study, we performed molecular characterization of genes encoding members of this protein family. Through cDNA sequencing, we constructed full-length gene models and verified genes that are protein coding and those that are pseudogenes. We also used quantitative PCR to measure their in vivo transcript abundances in clinical P. vivax isolates. Like genes encoding related invasion ligands of P. falciparum, Pvrbp expression levels vary broadly across different parasite isolates. Through antibody measurements, we found that host immune pressure may be the driving force behind the distinctly high diversity of one of the family members, PvRBP2c. Mild yet significant negative correlation was found between parasitemia and the PvRBP2b antibody level, suggesting that antibodies to the protein may interfere with invasion.