Cardiotoxicity of verrucotoxin, a protein isolated from the venom of Synanceia verrucosa
Cardiotoxicity of verrucotoxin, a protein isolated from the venom of Synanceia verrucosa
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DOI：
10.1016/s0041-0101(96)00075-x
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发表时间：
1997-01-01
期刊：
影响因子：
2.8
通讯作者：
Perriere, C
中科院分区：
文献类型：
--
作者：
Garnier, P;Sauviat, MP;Perriere, C
P. Garnier，M.- P. Sauviat，F. Goudey-Perriere和C.佩里耶疣毒素的毒性，一种从疣状合囊水母毒液中分离的蛋白质。Toxicon 35，47-55，1997年。新鲜纯化的，但不稳定的疣毒素（VTX）和一个更稳定的蛋白质复合物的毒素（p-VTX）的分离，从毒的石鱼Synanceia verrucosa和应用于青蛙心房纤维。VTX和p-VTX降低刺激峰张力的幅度和持续时间，加速收缩的松弛相。p-VTX的负性肌力作用随Ringer液中外源性钙浓度（[Ca]（o））的增加而减弱。p-VTX引起的负性变时效应对[Ca]（o）的变化不敏感。格列本脲可逆转该效应。p-VTX缩短动作电位的平台期和复极化相。格列本脲（而非四乙铵）可逆转p-VTX诱导的AP复极化期缩短。结果表明，从中华绒螯蟹毒液中分离出的毒素，其毒性与中华绒螯蟹的毒力有关. verrucosa抑制蛙心房肌Ca通道，并可能激活ATP敏感性钾通道。版权所有（C）1996 Elsevier Science Ltd.
P. Garnier, M.-P. Sauviat, F. Goudey-Perriere and C. Perriere. Cardiotoxicity of verrucotoxin, a protein isolated from the venom of Synanceia verrucosa. Toxicon 35, 47-55, 1997.-Freshly purified but unstable verrucotoxin (VTX) and a more stable proteic complex of the toxin (p-VTX) were isolated from the venom of the stonefish Synanceia verrucosa and applied to frog atrial fibres. VTX and p-VTX decreased the amplitude and the duration of the stimulated peak tension and accelerated the relaxation phase of the contraction. The negative inotropic effect of p-VTX decreased with increasing the external Ca concentration ([Ca](o)) in the Ringer solution. The negative chronotropic effect induced by p-VTX was insensitive to change in [Ca](o). It is reversed by glibenclamide. p-VTX shortened the duration of the plateau and the repolarizing phase of the action potential. Glibenclamide but not tetraethylammonium reversed the p-VTX-induced shortening of the AP repolarizing phase. The data suggest that the toxin isolated from the venom of S. verrucosa inhibits Ca channels and might activate ATP-sensitive potassium channels in frog atrial heart muscle. Copyright (C) 1996 Elsevier Science Ltd.