Systematic identification of chicken type I, II and III interferon-stimulated genes
Systematic identification of chicken type I, II and III interferon-stimulated genes
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鸡I、II、III型干扰素刺激基因的系统鉴定
DOI：
10.1186/s13567-020-00793-x
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发表时间：
2020-05-24
影响因子：
4.4
通讯作者：
Feng, Min
中科院分区：
文献类型：
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作者：
Dai, Manman;Xie, Tingting;Feng, Min
干扰素刺激基因（ISGs）在抗病毒先天免疫应答中发挥重要作用。尽管在哺乳动物中已经发现了许多isg，但研究人员普遍认为，还有更多的isg尚未被发现。目前的资料仍然非常有限，特别是在系统地确定第三类地下地震系统方面。同样，目前对鸟类isg的研究仍处于起步阶段。本研究旨在系统鉴定鸡I型（IFN-α）、II型（IFN-γ）和III型（IFN-λ） isg，并分析它们各自的应答因子。采用RNA测序技术（RNA- seq）鉴定IFN-α、IFN-γ和IFN-λ处理后表达上调的基因。在鸡中鉴定出I型ISGs 205个，II型ISGs 299个，III型ISGs 421个。我们进一步在ISGs的启动子区域寻找ifn刺激反应元件（ISRE）和γ激活序列（GAS）元件。GAS元件在II型ISGs的启动子中很常见，甚至在I型和III型ISGs中也检测到。然而，ISRE在鸡isg的启动子中并不常见。此外，我们证明了IFN-α或IFN-λ处理显著激活了鸡细胞中的ISRE，并且预期，IFN-γ处理也显著激活了GAS元件。有趣的是，我们还发现GAS元件被IFN-λ显著激活。我们的研究为鸡提供了一个系统的isg文库，并提供了鉴定出的isg转录调控的初步信息。
Interferon-stimulated genes (ISGs) play an important role in antiviral innate immune responses. Although many ISGs have been identified in mammals, researchers commonly recognize that many more ISGs are yet to be discovered. Current information is still very limited particularly for the systematic identification of type III ISGs. Similarly, current research on ISGs in birds is still in its infancy. The aim of this study was to systematically identify chicken type I (IFN-α), II (IFN-γ) and III (IFN-λ) ISGs and analyze their respective response elements. RNA sequencing (RNA-Seq) was employed to identify those genes with up-regulated expression following chicken IFN-α, IFN-γ and IFN-λ treatment. Two hundred and five type I ISGs, 299 type II ISGs, and 421 type III ISGs were identified in the chicken. We further searched for IFN-stimulated response elements (ISRE) and gamma-activated sequences (GAS) elements in the promoters region of ISGs. The GAS elements were common in the promoter of type II ISGs and were even detected in type I and III ISGs. However, ISRE were not commonly found in the promoters of chicken ISGs. Furthermore, we demonstrated that ISRE in chicken cells were significantly activated by IFN-α or IFN-λ treatment, and expectedly, that GAS elements were also significantly activated by IFN-γ treatment. Interestingly, we also found that GAS elements were significantly activated by IFN-λ. Our study provides a systematic library of ISGs in the chicken together with preliminary information about the transcriptional regulation of the identified ISGs.