The PPR protein SLOW GROWTH 4 is involved in editing of nad4 and affects the splicing of nad2 intron 1
The PPR protein SLOW GROWTH 4 is involved in editing of nad4 and affects the splicing of nad2 intron 1
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DOI：
10.1007/s11103-016-0566-4
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发表时间：
2016-12
影响因子：
5.1
通讯作者：
Stefan Weißenberger;J. Soll;Chris Carrie
中科院分区：
文献类型：
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作者：
Stefan Weißenberger;J. Soll;Chris Carrie
eslo4是一种线粒体PPR蛋白，参与nad4的编辑，可能是nad2内含子1的有效剪接所必需的。摘要五肽重复（pentatricopeptide repeat， PPR）蛋白是开花植物中一个庞大的蛋白家族，被认为主要参与细胞器RNA代谢。发现pls型PPR蛋白亚群是胞苷对尿苷RNA编辑的主要特异性因子。确定pls型PPR蛋白的靶标有助于阐明细胞器基因组中编码蛋白的分子功能。本研究对缺乏SLOW GROWTH 4 PPR蛋白的植物进行了表征。slo4突变体的特点是根系生长受限，开花晚，整体生长延迟。突变植株中线粒体复合体I的蛋白质水平和活性降低，推测的复合体I组装中间体积累。在线粒体nad4转录本中发现了一个编辑缺陷，导致氨基酸变化，编码一个复合体I亚基。此外，编码另一个复合体I亚基的nad2的第一个内含子的剪接效率也降低了。然而，剪接效率的变化不能与转录本中的任何编辑缺陷联系起来。
Key messageSLO4 is a mitochondrial PPR protein that is involved in editingnad4, possibly required for the efficient splicing ofnad2intron1.AbstractPentatricopeptide repeat (PPR) proteins constitute a large protein family in flowering plants and are thought to be mostly involved in organellar RNA metabolism. The subgroup of PLS-type PPR proteins were found to be the main specificity factors of cytidine to uridine RNA editing. Identifying the targets of PLS-type PPR proteins can help in elucidating the molecular function of proteins encoded in the organellar genomes. In this study, plants lacking the SLOW GROWTH 4 PPR protein were characterized.Slo4mutants were characterized as having restricted root growth, being late flowering and displaying an overall delayed growth phenotype. Protein levels and activity of mitochondrial complex I were decreased and putative complex I assembly intermediates accumulated in the mutant plants. An editing defect, leading to an amino acid change, in the mitochondrialnad4transcript, encoding for a complex I subunit, was identified. Furthermore, the splicing efficiency of the first intron ofnad2, encoding for another complex I subunit, was also decreased. The change in splicing efficiency could however not be linked to any editing defects in thenad2transcript.