Macrophages stimulate DNA synthesis in rat alveolar type II cells.
Macrophages stimulate DNA synthesis in rat alveolar type II cells.
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巨噬细胞刺激大鼠 II 型肺泡细胞的 DNA 合成。
DOI：
10.1164/arrd.1985.132.6.1246
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Mason,RJ
中科院分区：
文献类型：
--
作者：
Leslie,CC;McCormick-Shannon,K;Cook,JL;Mason,RJ
肺损伤后肺泡II型细胞的增殖对上皮的修复至关重要。由于巨噬细胞的涌入是与急性肺损伤相关的炎症反应的一部分，并且巨噬细胞对多种细胞类型产生有丝分裂因子，因此进行了实验以确定巨噬细胞是否刺激II型细胞的DNA合成。由巨噬细胞调节的透析培养基持续刺激II型细胞的DNA合成，而由各种其他细胞类型调节的培养基则没有。我们实验室生产的sv40转化的巨噬细胞系也能分泌增强3h -胸腺嘧啶并入II型细胞的物质。此外，大鼠肺泡巨噬细胞与II型细胞共培养可刺激上皮细胞的DNA合成。放射自显影显示，在塑料或内皮细胞细胞外基质上培养的II型细胞中加入巨噬细胞，上皮细胞的标记指数分别从1%提高到15%和从19%提高到51%。促进DNA合成增加和细胞数量增加的培养条件是，将II型细胞置于含有巨噬细胞条件培养基、霍乱毒素、胰岛素和表皮生长因子的细胞外基质上。提示巨噬细胞分泌的物质在体内调节肺泡II型细胞增殖中发挥作用。
Proliferation of alveolar type II cells after lung injury is crucial for repair of the epithelium. Because an influx of macrophages occurs as part of the inflammatory response associated with acute lung injury and macrophages produce mitogenic factors for a variety of cell types, experiments were conducted to determine if macrophages stimulated DNA synthesis in type II cells. Dialyzed medium conditioned by macrophages consistently stimulated type II cell DNA synthesis, whereas medium conditioned by a variety of other cell types did not. A SV40-transformed macrophage cell line, produced in our laboratory, also secreted substance(s) that enhanced3H-thymidine incorporation into type II cells. In addition, coculturing rat alveolar macrophages with type II cells stimulated DNA synthesis in the epithelial cells. As determined by autoradiography, the addition of macrophages to type II cells cultured on plastic or on an endothelial cell extracellular matrix increased the labeling index of the epithelial cells from 1 to 15% and from 19 to 51%, respectively. The culture conditions that promoted the greatest increase in DNA synthesis, as well as an increase in cell number, occurred with type II cells plated on an extracellular matrix in medium containing macrophage-conditioned medium, cholera toxin, insulin, and epidermal growth factor. The results suggest that substances secreted by macrophages play a role in regulating alveolar type II cell proliferationin vivo.