1α,25(OH)2 Vitamin D3 Modulates Avian T Lymphocyte Functions without Inducing CTL Unresponsiveness.
1α,25(OH)2 Vitamin D3 Modulates Avian T Lymphocyte Functions without Inducing CTL Unresponsiveness.
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1α，25（OH）2维生素D3调节鸟类T淋巴细胞功能，而不会引起CTL无反应性。
DOI：
10.1371/journal.pone.0150134
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Behboudi S
中科院分区：
文献类型：
--
作者：
Boodhoo N;Sharif S;Behboudi S
1，25-二羟基维生素D3（维生素D）是一种天然合成的脂溶性维生素，在人类和小鼠模型中显示具有免疫调节，抗炎和预防癌症的特性。本研究采用鸡干扰素（IFN）-γ ELISPOT法、BrdU增殖法、Annexin V凋亡法和PhosFlow检测磷酸化信号分子的方法，研究了维生素D对禽类T淋巴细胞功能的影响。结果表明，维生素D能显著抑制T淋巴细胞产生IFN-γ和增殖（P≤0.05），但保留了T淋巴细胞脱颗粒的能力，而脱颗粒是T淋巴细胞毒性的标志。类似地，维生素D在刺激的T淋巴细胞群体中不抑制细胞外信号调节激酶（ERK）1/2磷酸化，这是T细胞信号传导的关键介质，而在未刺激的细胞中降低ERK 1/2磷酸化水平。我们的数据提供的证据表明，维生素D对鸡T淋巴细胞具有免疫调节特性，而不会诱导无反应性，并通过限制免疫病理学可以促进对家禽传染病的保护性免疫。
1,25-Dihydroxyvitamin D3 (Vitamin D) is a naturally synthesized fat soluble vitamin shown to have immunomodulatory, anti-inflammatory and cancer prevention properties in human and murine models. Here, we studied the effects of Vitamin D on the functional abilities of avian T lymphocytes using chicken Interferon (IFN)-γ ELISPOT assay, BrdU proliferation assay, Annexin V apoptosis assay and PhosFlow for detecting phosphorylated signalling molecules. The results demonstrate that Vitamin D significantly inhibited the abilities of T lymphocytes to produce IFN-γ and proliferate in vitro (P≤0.05), but retained their ability to undergo degranulation, which is a maker for cytotoxicity of these cells. Similarly, Vitamin D did not inhibit Extracellular signal-Regulated Kinase (ERK) 1/2 phosphorylation, a key mediator in T cell signalling, in the stimulated T lymphocytes population, while reduced ERK1/2 phosphorylation levels in the unstimulated cells. Our data provide evidence that Vitamin D has immuno-modulatory properties on chicken T lymphocytes without inducing unresponsiveness and by limiting immuno-pathology can promote protective immunity against infectious diseases of poultry.