Lipid droplet dynamics during Schizosaccharomyces pombe sporulation and their role in spore survival.
Lipid droplet dynamics during Schizosaccharomyces pombe sporulation and their role in spore survival.
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DOI：
10.1242/bio.022384
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发表时间：
2017-02-15
期刊：
影响因子：
2.4
通讯作者：
Hiraoka Y
中科院分区：
文献类型：
--
作者：
Yang HJ;Osakada H;Kojidani T;Haraguchi T;Hiraoka Y
在氮饥饿时，裂殖酵母裂殖酵母形成休眠孢子;然而，孢子在没有获得外源营养的情况下维持生命的机制仍然不清楚。脂滴是中性脂质的储库，其充当重要的细胞能量资源。使用活细胞成像分析，我们发现母细胞的脂滴重新分配到它们的新生孢子中。值得注意的是，这一过程是肌动蛋白聚合依赖和促进的前缘蛋白的前孢子膜。缺乏三酰甘油合成的孢子不能萌发，而三酰甘油是形成脂滴所必需的。我们的研究结果表明，脂滴是重要的孢子的生命维持。总结：酵母母细胞的脂滴显示重新分配到他们的新生孢子活细胞成像分析，表明脂滴是重要的酵母孢子的生存。
Upon nitrogen starvation, the fission yeast Schizosaccharomyces pombe forms dormant spores; however, the mechanisms by which a spore sustains life without access to exogenous nutrients remain unclear. Lipid droplets are reservoirs of neutral lipids that act as important cellular energy resources. Using live-cell imaging analysis, we found that the lipid droplets of mother cells redistribute to their nascent spores. Notably, this process was actin polymerization-dependent and facilitated by the leading edge proteins of the forespore membrane. Spores lacking triacylglycerol synthesis, which is essential for lipid droplet formation, failed to germinate. Our results suggest that the lipid droplets are important for the sustenance of life in spores. Summary: Lipid droplets of yeast mother cells are shown to redistribute to their nascent spores by live-cell imaging analysis, suggesting that the lipid droplets are important for yeast spore survival.