Characterisation of Yersinia pestis isolates from natural foci of plague in the Republic of Georgia, and their relationship to Y. pestis isolates from other countries.
Characterisation of Yersinia pestis isolates from natural foci of plague in the Republic of Georgia, and their relationship to Y. pestis isolates from other countries.
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格鲁吉亚共和国鼠疫自然疫源地鼠疫耶尔森氏菌分离株的特征及其与其他国家鼠疫耶尔森氏菌分离株的关系。
DOI：
10.1111/j.1469-0691.2008.01953.x
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
Sulakvelidze,A
中科院分区：
文献类型：
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作者：
Revazishvili,T;Rajanna,C;Bakanidze,L;Tsertsvadze,N;Imnadze,P;O'Connell,K;Kreger,A;Stine,OC;MorrisJr,JG;Sulakvelidze,A
分析了来自格鲁吉亚共和国鼠疫地方病疫源地的40株鼠疫耶尔森菌和来自邻近的前苏联国家的6株鼠疫耶尔森菌的生化和表型特性，并通过脉冲场凝胶电泳（PFGE）比较了它们与鼠疫耶尔森菌菌株KIM和CO92的遗传相关性。此外，基于16S rRNA、hsp60、glnA、gyrB、recA、manB、thrA和tmk基因座的序列数据，采用多位点序列分型（MLST）技术，将来自美国的11株鼠疫耶尔森氏菌，以及已发表的鼠疫耶尔森氏菌菌株KIM、CO92和91001的核苷酸序列与目前收集的46株分离株进行了比较。对4个毒力基因位点（caf1、lcrV、psaA和pla）进行了测序分析。两个序列类型（ST 1和ST 2），这是由一个单一的核苷酸不同，由MLST确定。除一株分离株（771G）外，所有格鲁吉亚鼠疫杆菌均属于ST 2。PFGE还将格鲁吉亚鼠疫耶尔森氏菌分离株与非格鲁吉亚分离株分开分组。总的来说，PFGE比MLST更有效地区分了鼠疫杆菌。在所有格鲁吉亚和非格鲁吉亚分离株中，4个毒力基因中的3个（lcrV、psaA和pla）的序列是相同的，但af 1位点由两种等位基因类型代表，其中caf1NT 1与非格鲁吉亚分离株相关，caf1NT 2与格鲁吉亚分离株相关。这些结果表明，格鲁吉亚鼠疫菌是克隆起源。
FortyYersinia pestisisolates from endemic foci of plague in the Republic of Georgia, and sixY. pestisisolates from neighbouring former Soviet Union countries, were analysed for their biochemical and phenotypic properties, and their genetic relatedness was compared withY. pestisstrains KIM and CO92 by pulsed‐field gel electrophoresis (PFGE). In addition, 11Y. pestisisolates from the USA, together with published nucleotide sequences fromY. pestisstrains KIM, CO92 and 91001, were compared with the 46 isolates in the present collection using multilocus sequence typing (MLST), based on sequence data for the 16S rRNA,hsp60,glnA,gyrB,recA,manB,thrAandtmkloci. Four virulence gene loci (caf1,lcrV,psaAandpla) were also sequenced and analysed. Two sequence types (ST1 and ST2), which differed by a single nucleotide, were identified by MLST. With the exception of a single isolate (771G), all of the GeorgianY. pestisisolates belonged to ST2. PFGE also grouped the GeorgianY. pestisisolates separately from the non‐Georgian isolates. Overall, PFGE discriminated theY. pestisisolates more effectively than MLST. The sequences of three of the four virulence genes (lcrV,psaAandpla) were identical in all Georgian and non‐Georgian isolates, but thecaf1locus was represented by two allele types, withcaf1NT1 being associated with the non‐Georgian isolates andcaf1NT2 being associated with the Georgian isolates. These results suggest that GeorgianY. pestisisolates are of clonal origin.