A reevaluation of fatty acids as inflammatory agents in acne.
A reevaluation of fatty acids as inflammatory agents in acne.
复制标题
重新评估脂肪酸作为痤疮炎症剂的作用。
DOI：
10.1111/1523-1747.ep12491639
复制
发表时间：
1977
期刊：
影响因子：
--
通讯作者：
M. Sakamoto
中科院分区：
文献类型：
--
作者：
S. M. Puhvel;M. Sakamoto
目前广泛持有的理论是，卵泡内游离脂肪酸（FA）是痤疮炎症变化的主要诱因是基于间接证据。没有直接证据表明生理浓度的FA具有炎症性。在本研究中，分析了游离毛囊皮脂腺导管和游离粉刺中FA的含量。利用这些数值，研究了FA对人皮肤皮内注射的影响。10例男性上背皮肤257个毛囊皮脂腺的FA范围为0.19 ~ 2.43马克杯，平均每条毛囊的FA为0.89±0.75马克杯。45个秃发的平均FA含量为63.6 +/- 24.8马克/秃发。用于皮内试验的脂肪酸是从人皮肤表面脂质和从分离的皮脂腺中纯化的水解甘油三酯中分离出来的。26名受试者在上背部静脉注射100杯FA。对FA注射的反应不能与生理盐水对照注射的反应区分开来。24小时后，所有受试者的皮肤均未出现红斑、硬化或任何可见的炎症痕迹。在组织学水平上，FA注射部位以淋巴细胞为主的轻度炎症浸润比生理盐水对照注射部位更明显。将FA的注射量增加到500杯，在人体皮肤中仍然没有产生明显的炎症反应。我们的结论是，皮肤内注射生理浓度的FA不会在人体皮肤中产生非常轻微的炎症反应，并表明痤疮丙酸杆菌在痤疮发病过程中的作用可能比仅仅作为滤泡内脂肪酶的来源更为复杂。
The currently widely held theory that intrafollicular free fatty acids (FA) are the primary agents instigating inflammatory changes in acne is based on circumstantial evidence. There is no direct evidence that FA in physiologic concentrations are inflammatory. In the present study the quantities of FA present in isolated pilosebaceous ducts and in isolated comedones were analyzed. Using these values, the effect of FA on intracutaneous injection into human skin was investigated. The range of FA in 257 isolated pilosebaceous ducts from skin of the upper back of 10 male subjects was 0.19 to 2.43 mug, with an average of 0.89 +/- 0.75 mug of FA per duct. The mean FA content in 45 open comedones was 63.6 +/- 24.8 mug per comedone. Fatty acids for intracutaneous testing were isolated from human skin surface lipids and from hydrolyzed triglycerides purified from pooled isolated sebaceous glands. Twenty-six subjects received 100 mug of FA intracutaneously in the upper back. The response to FA injections could not be distinguished from the response to saline control injections. By 24 hr no erythema, induration, or any visible marks of inflammation were present in the skin of any of the subjects tested. At the histologic level a mild inflammatory infiltrate consisting perdominantly of lymphocytes was slightly more marked in the FA injection site than in the saline control injection site. Increasing the amount of FA injected to 500 mug still produced no visible inflammatory response in human skin. We conclude that intracutaneous injections of FA in physiologic concentrations do not produce more than a very mild inflammatory reaction in human skin and suggest that the role of Propionibacterium acnes in the pathogeneisis of acne may be more complex than merely as a source of intrafollicular lipases.