Indirect genetic effects from ecological interactions in Arabidopsis thaliana
Indirect genetic effects from ecological interactions in Arabidopsis thaliana
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DOI：
10.1111/j.1365-294x.2007.03259.x
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发表时间：
2007-06-01
影响因子：
4.9
通讯作者：
Wolf, Jason B.
中科院分区：
文献类型：
--
作者：
Mutic, Joshua J.;Wolf, Jason B.
当一个个体的基因表达影响其他个体的性状表达时，就会产生间接遗传效应。间接遗传效应的重要性在包括亲缘选择、性选择、群落结构和多层次选择在内的多种进化过程中得到了认可，但关于它们的遗传结构和在整个基因组中的流行程度的数据仍然很少，特别是关于不相关个体之间相互作用的数据。利用411个Bay-0 x Shahdara拟南芥重组自交系与Landsberg邻居一起培养，研究了对大小、发育和适合度相关性状有直接和间接影响的数量性状位点（QTL）。利用区间定位方法，我们鉴定出15个具有直接影响的QTL，发现其中13个QTL对邻近植物的性状表达具有显著的间接影响。这些结果表明广泛的多效性，因为几乎所有的直接效应QTL都有相关的多效性间接效应。矛盾的是，大多数间接效应与直接效应具有相同的标志，创造了一种几乎普遍的正多效性模式，使直接效应和间接效应之间的大多数协方差为正。这些结果与种内相互作用的复杂遗传基础一致，但表明邻近植物之间的相互作用在很大程度上是积极的，而不是像竞争中预期的那样是消极的。除了在进化和生态方面的重要性外，DGE和IGE位点之间的多效性关系对自然种群的定量遗传研究以及实验设计的考虑也具有重要意义。此外，忽略IGEs的研究可能会高估或低估定量遗传参数，以及QTL的影响和方差。
Indirect genetic effects arise when genes expressed in one individual affect the expression of traits in other individuals. The importance of indirect genetic effects has been recognized for a diversity of evolutionary processes including kin selection, sexual selection, community structure and multilevel selection, but data regarding their genetic architecture and prevalence throughout the genome remain scarce, especially for interactions between unrelated individuals. Using a set of 411 Bay-0 x Shahdara Arabidopsis recombinant inbred lines grown with Landsberg neighbours, we examined quantitative trait loci (QTL) having direct and indirect effects on size, developmental, and fitness related traits. Using an interval mapping approach, we identified 15 QTL with direct effects and found that 13 of these QTL had significant indirect effects on trait expression in neighbouring plants. These results suggest widespread pleiotropy, as nearly all direct effect QTL have associated pleiotropic indirect effects. Paradoxically, most indirect effects were of the same sign as direct effects, creating a pattern of nearly universal positive pleiotropy that makes most covariances between direct and indirect effects positive. These results are consistent with a complex genetic basis for intraspecific interactions, but suggest that interactions between neighbouring plants are largely positive, rather than negative as would be expected for competition. In addition to their evolutionary and ecological importance, these pleiotropic relationships between DGE and IGE loci have implications for quantitative genetic studies of natural populations as well as experimental design considerations. Additionally, studies that ignore IGEs may over- or underestimate quantitative genetic parameters, as well as the effect of and variance contributed by QTL.