Vitamin A equivalence of spirulina β-carotene in Chinese adults as assessed by using a stable-isotope reference method
Vitamin A equivalence of spirulina β-carotene in Chinese adults as assessed by using a stable-isotope reference method
复制标题
DOI：
10.1093/ajcn/87.6.1730
复制
发表时间：
2008-06-01
影响因子：
7.1
通讯作者：
Yin, Shian
中科院分区：
文献类型：
--
作者：
Wang, Jie;Wang, Yin;Yin, Shian
背景资料：螺旋藻是一种高蛋白的食品补充剂，含有类胡萝卜素。目的：本研究的目的是确定维生素A当量的螺旋藻β-carotene in humans.Design：螺旋藻生长在23原子%（H2O）-H-2文化解决方案。螺旋藻β-胡萝卜素以[H-2（10）]反式β-胡萝卜素的富集程度最高。10名健康中国男性，平均（+/- SD）血清视黄醇浓度为1.7 +/- 0.3 μ mol/L，体重指数（kg/m2）为23 +/- 3，早餐含13 g脂肪，食用5.8 μ mol [C-13（10）]醋酸视黄酯油作为参考剂量。一周后，每名受试者食用7.9 μ mol反式β-胡萝卜素的螺旋藻与早餐含有22克脂肪。所有受试者都遵循低类胡萝卜素和维生素A的饮食。在56天的时间内从每个受试者收集40份血液样本。结果：螺旋藻β-胡萝卜素与维生素A的平均转换系数为4.5 ± 1.6（范围：2.3-6.9），与[C-13（10）]维生素A醋酸酯剂量的血清反应相比，血清中维生素A和β-胡萝卜素的浓度和富集度均显著增加。据估计，80%的转换发生在螺旋藻administration.Conclusion后的第一个24小时内：在一组营养良好，体重正常的中国男性低维生素A饮食，4.5毫克螺旋藻β-胡萝卜素消耗22克脂肪具有相同的维生素A的活性作为1毫克维生素A乙酸酯。
Background: Spirulina is a high-protein food supplement that contains carotenoids.Objective: The objective of the study was to determine the vitamin A equivalence of spirulina beta-carotene in humans.Design: Spirulina was grown in a 23 atom% (H2O)-H-2 cultural solution. Spirulina P-carotene showed the greatest enrichment as [H-2(10)]trans beta-carotene. Ten healthy Chinese men with a mean (+/- SD) serum retinol concentration of 1.7 +/- 0.3 mu mol/L and a body mass index (in kg/m(2)) of 23 +/- 3 consumed 5.8 mu mol [C-13(10)]retinyl acetate in oil as a reference dose with a breakfast containing 13 g fat. One week later, each subject consumed 7.9 mu mol trans beta-carotene in spirulina with a breakfast containing 22 g fat. All subjects followed diets low in carotenoid and vitamin A. Forty blood samples were collected from each subject over a span of 56 d. Concentrations and enrichments of retinol and beta-carotene in serum samples were determined by using HPLC and a mass spectrometer.Results: Compared with the serum response to [C-13(10)]retinyl acetate dose, the mean conversion factor of spirulina beta-carotene to retinol was 4.5 +/- 1.6 (range: 2.3-6.9) by weight. It was estimated that 80% of the conversion occurred within the first 24 h after spirulina administration.Conclusion: In a group of well-nourished, normal-weight Chinese men following low-vitamin A diets, 4.5 mg spirulina beta-carotene consumed with 22 g fat has the same vitamin A activity as does 1 mg retinyl acetate.