Cholinergic mediation of spatial memory in the preweanling rat: application of the radial arm maze paradigm.
Cholinergic mediation of spatial memory in the preweanling rat: application of the radial arm maze paradigm.
复制标题
断奶前大鼠空间记忆的胆碱能介导：径向臂迷宫范式的应用。
作者：
S. Rauch;L. A. Raskin
这些实验通过桡臂迷宫范式来评估发育中的大鼠的空间记忆能力。实验对象从16天开始在迷宫中接受训练和测试，直到他们25天大。结果显示，接受过训练的动物比那些对任务没有经验的动物表现得好得多，也比偶然的好，这表明它们具有这种学习的早期能力。第二个实验通过桡臂迷宫研究了这个年龄段空间记忆的神经机制。为了区分任务的工作记忆部分和参考记忆部分，对基本的径向臂迷宫范式进行了改进。从16天开始训练的受试者在25天测试前接受生理盐水、甲基东莨菪碱、东莨菪碱或槟榔碱的药物注射。结果表明，中枢胆碱能拮抗剂严重损害工作记忆，保留参考记忆。这一发现与现有文献中乙酰胆碱在成人学习和记忆，特别是工作记忆中的作用是一致的。最重要的是，这些实验证明：(a)桡臂迷宫范式可以有效地用于大鼠学习和记忆的发育研究；(b)胆碱能系统在早期调节工作记忆。
These experiments assessed spatial memory capabilities in the developing rat by the radial arm maze paradigm. Subjects were trained and tested in the maze beginning at 16 days until they were 25 days of age. Results showed that animals that received training performed significantly better than those naive to the task, and better than chance, which suggests an early capacity for this type of learning. The second experiment investigated the neural mechanism that underlies spatial memory at this age as measured by the radial arm maze. In order to distinguish between the working and the reference memory components of the task, a modification of the basic radial arm maze paradigm was used. Subjects trained from 16 days received drug injections of saline, methylscopolamine, scopolamine, or arecoline prior to testing at age 25 days. Results indicated that central cholinergic antagonism severely impairs working memory while sparing reference memory. This finding is consistent with the existing literature that suggests a role for acetylcholine in adult learning and memory, specifically in working memory. Most important, these experiments document that (a) the radial arm maze paradigm can be used effectively for the developmental study of learning and memory in the rat and (b) cholinergic system(s) mediate working memory at an early age.