Functional dissection of the paired segmentation gene in Drosophila embryos.
Functional dissection of the paired segmentation gene in Drosophila embryos.
复制标题
果蝇胚胎中配对分割基因的功能解剖。
DOI：
10.1101/gad.5.9.1684
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发表时间：
1991
影响因子：
10.5
通讯作者：
Weir,M
中科院分区：
文献类型：
--
作者：
Morrissey,D;Askew,D;Raj,L;Weir,M
利用果蝇胚胎异位表达实验研究了成对规则分割基因在片段极性基因enrailed（en）空间调控中的作用。假设两个基因的表达重叠区域，配对（prd）和偶跳（eve），定义了奇数的en表达条纹。与这种组合模型一致，prd的异位表达导致这些en条纹向后扩展。然而，令人惊讶的是，缺失保守配对盒的prd基因的异位表达导致这些奇数条的丢失。这种显性负效应是PRD胚胎中EN表达的表型，并表明配对盒对正常PRD功能是必要的。一个类似的删除奇数EN条纹后，也观察到异位表达的嵌合fushi tarazu（FTZ）基因含有一个替代的PRD基因同源盒;此外，在这些胚胎中，偶数EN条纹向前扩展，观察时，未改变的FTZ基因异位表达。这些作用表明，嵌合蛋白可能具有正常Ftz和Prd蛋白的DNA或蛋白质靶标。
An ectopic expression assay in Drosophila embryos was used to investigate the roles of pair-rule segmentation genes in the spatial regulation of the segment-polarity gene, engrailed (en). It is hypothesized that the regions of overlap in expression of two genes, paired (prd) and even-skipped (eve), define the odd-numbered en expression stripes. Consistent with this combinatorial model, ectopic expression of prd caused these en stripes to be expanded posteriorly. Surprisingly, however, ectopic expression of a prd gene with a deletion of the conserved paired box resulted in loss of these odd-numbered en stripes. This dominant negative effect is a phenocopy of en expression in prd embryos and suggests that the paired box is necessary for normal prd- function. A similar deletion of odd-numbered en stripes was also observed after ectopic expression of a chimeric fushi tarazu (ftz) gene containing a substituted prd gene homeo box; in addition, in these embryos, the even-numbered en stripes were expanded anteriorly, as observed when the unaltered ftz gene is ectopically expressed. These effects suggest that the chimeric protein may have DNA or protein targets of both the normal Ftz and Prd proteins.