Endogenous phospholipid metabolism in stimulated neutrophils differential activation by FMLP and PMA.
Endogenous phospholipid metabolism in stimulated neutrophils differential activation by FMLP and PMA.
复制标题
FMLP 和 PMA 刺激中性粒细胞的内源磷脂代谢差异激活。
DOI：
10.1016/0006-291x(82)90615-5
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发表时间：
1982
影响因子：
3.1
通讯作者：
Weissmann,G
中科院分区：
文献类型：
--
作者：
Serhan,CN;Broekman,MJ;Korchak,HM;Marcus,AJ;Weissmann,G
在中性粒细胞（PMN）刺激的初始0-120秒内检测内源性磷脂代谢。当PMN暴露于趋化肽FMLP（10−7M）或肿瘤启动子肉豆酸酯磷（PMA, 1 μg/ml）时，特定磷脂（PL）类别在15秒内发生广泛变化。刺激诱导的PL变化的特征和动力学依赖于刺激。添加FMLP 5 s后，PC、PS和PA的PMN含量增加，PI含量降低。动力学研究表明，只有PA水平保持升高（0-120 s），而其他PL水平下降。当PMA浓度为1 μg/ml时，细胞中PC和PS的水平迅速升高（< 15 s）。在PMA刺激下，直到60 ~ 60 s才观察到PI和PA的变化。结果表明，PMN暴露于刺激下会导致特定PL的快速变化。此外，它们支持中性粒细胞在刺激下迅速“重塑”内源性PL的概念。
Endogenous phospholipid metabolism was examined during the initial 0–120 seconds of neutrophil (PMN) stimulation. When PMN were exposed to the chemotactic peptide FMLP (10−7M) or the tumor promotor, phorbol myristate acetate (PMA, 1 μg/ml) extensive changes in specific phospholipid (PL) classes were evident within 15 seconds. The profile and kinetics of stimulus-induced PL changes were stimulus-dependent. Five seconds after the addition of FMLP, PMN content of PC, PS and PA increased, while the level of PI decreased. Kinetic studies revealed that only PA levels remained elevated (0–120 s) while other PL decreased. In contrast, when cells were exposed to PMA (1 μg/ml), the levels of PC and PS rapidly increased (< 15 s). With PMA as stimulus, changes in PI and PA were not observed until > 60 s. Results indicate that exposure to PMN to stimuli leads to rapid changes in specific PL. In addition, they support the concept that neutrophils rapidly “remodel” endogenous PL upon stimulation.