Antibiotic treatment disrupts bacterial communities in the colon and rectum of simian immunodeficiency virus-infected macaques
Antibiotic treatment disrupts bacterial communities in the colon and rectum of simian immunodeficiency virus-infected macaques
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DOI：
10.1093/femsle/fnx228
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发表时间：
2017-12-01
影响因子：
2.1
通讯作者：
Klatt, Nichole R.
中科院分区：
文献类型：
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作者：
Zevin, Alexander S.;Hensley-McBain, Tiffany;Klatt, Nichole R.
众所周知，抗生素疗法会破坏胃肠道 (GI) 细菌群落。 HIV 和致病性猿猴免疫缺陷病毒 (SIV) 感染也与胃肠道细菌群落破坏有关。我们对六只感染 SIV 的恒河猴进行联合抗生素治疗，并在使用抗生素之前和之后收集结肠活检、粪便样本和直肠拭子，并使用高通量 16S rRNA 基因测序评估每个样本点的细菌群落。结肠粘膜和粪便样本显示出不同的细菌群落，而直肠拭子则显示出粘膜和粪便相关细菌的混合物。抗生素破坏了每个样本点的本地细菌群落。结肠粘膜显示出优势螺杆菌科的丰度减少，而我们发现优势瘤胃球菌科的丰度减少。在凳子里。直肠拭子显示出与结肠粘膜相似的趋势，但变化更大。抗生素处理后，每个样本点的类似兼性厌氧菌类群（包括乳杆菌科和肠杆菌科）的丰度增加。
Antibiotic therapies are known to disrupt gastrointestinal (GI) bacterial communities. HIV and pathogenic simian immunodeficiency virus (SIV) infections have also been associated with disrupted GI bacterial communities. We administered a combination antibiotic therapy to six SIV-infected rhesus macaques and collected colon biopsies, stool samples and rectal swabs before and after antibiotics, and evaluated the bacterial communities at each sample site using high-throughput 16S rRNA gene sequencing. The colon mucosa and stool samples displayed different bacterial communities, while the rectal swabs showed a mixture of the mucosal and stool-associated bacteria. Antibiotics disrupted the native bacterial communities at each sample site. The colon mucosa showed depleted abundances of the dominant Helicobacteraceae, while we found depleted abundances of the dominant Ruminococcaceae sp. in the stool. The rectal swabs showed similar trends as the colon mucosa, but were more variable. After the antibiotic treatment, there were increased abundances of similar taxa of facultative anaerobic bacteria, including Lactobacillaceae and Enterobacteriaceae at each sample site.