Support for a proposed retinoid-processing protein complex in apical retinal pigment epithelium
Support for a proposed retinoid-processing protein complex in apical retinal pigment epithelium
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DOI：
10.1016/j.exer.2004.04.001
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发表时间：
2004-09-01
影响因子：
3.4
通讯作者：
Crabb, JW
中科院分区：
文献类型：
--
作者：
Bonilha, VL;Bhattacharya, SK;Crabb, JW
视网膜色素上皮 (RPE) 微粒体中细胞视黄醛结合蛋白 (CRALBP) 与 ERM（ezrin、radixin、moesin）结合磷蛋白 50 (EBP50) 的相互作用导致了这样的假设：顶端 RPE 中存在类视黄醇加工蛋白复合物。小鼠 RPE 顶端突起在麦芽凝集素包被的琼脂糖珠上分离。对分离的顶端 RPE 的蛋白质组学分析表明存在 CRALBP、EBP50、11-顺式视黄醇脱氢酶、细胞视黄醇结合蛋白 1 和光感受器间视黄醇结合蛋白。结果支持视觉周期蛋白复合物可能参与顶端 RPE 中 11-cis-retinoid 的定位和释放的假设。 (C) 2004 Elsevier Ltd. 保留所有权利。
The interaction of cellular retinaldehyde-binding protein (CRALBP) with ERM (ezrin, radixin, moesin)-binding phosphoprotein 50 (EBP50) in retinal pigment epithelium (RPE) microsomes has led to the hypothesis that a retinoid-processing protein complex exists in apical RPE. Mouse RPE apical processes were isolated on wheat germ agglutinin-coated agarose beads. Proteomic analyses of the isolated apical RPE demonstrated the presence of CRALBP, EBP50, 11-cis-retinol dehydrogenase, cellular retinol-binding protein 1, and interphotoreceptor retinoid-binding protein. The results support the hypothesis that a visual cycle protein complex may serve in the localization and release of 11-cis-retinoid in the apical RPE. (C) 2004 Elsevier Ltd. All rights reserved.