Acute ethanol intoxication regulates f-met-leu-phe-induced chemotaxis and superoxide release by neutrophils and Kupffer cells through modulation of the formyl peptide receptor in the rat.
Acute ethanol intoxication regulates f-met-leu-phe-induced chemotaxis and superoxide release by neutrophils and Kupffer cells through modulation of the formyl peptide receptor in the rat.
复制标题
急性乙醇中毒通过调节大鼠中的甲酰基肽受体来调节 f-met-leu-phe 诱导的中性粒细胞和 Kupffer 细胞的趋化性和超氧化物释放。
DOI：
10.1016/0024-3205(94)90161-9
复制
发表时间：
1994
期刊：
影响因子：
6.1
通讯作者：
Elliott,KE
中科院分区：
文献类型：
--
作者：
Bautista,AP;Elliott,KE
This study was performed to assess alcohol-induced alterations in superoxide release and chemotaxis by Kupffer cells and blood neutrophils. Male Sprague-Dawley rats received a bolus injection of alcohol (1.75 g/Kg) followed by an intravenous infusion (250–300 mg/Kg/hr). Three or 24 hr after alcohol infusion, blood neutrophils and Kuppfer cells were isolated and assayed for f-met-leu-phe-induced chemotaxis and superoxide release, and formyl peptide receptor expression. At 3 hr post-ethanol, f-met-leu-phe-induced-chemotaxis and superoxide release by blood neutrohils were increased 2 and 3-fold, compared to saline-treated group, and were further increased at 24 hr. The expression of formyl peptide receptors was also increased from 65, 000 ± 8, 000 sites per cell to 120, 000 ± 13,000 and 200,000 ± 16,400 sites at 3 and 24 hr post-ethanol, respectively. The equilibrium dissociation constant (KD) of these receptors on neutrophils was increased at the same time interval. In contrast, alcohol infusion for 3 hr attenuated f-met-leu-phe-induced superoxide release by Kupffer cells (0.8 ± 0.25 nmol/106cells), compared to saline-treated rats (3.7 ± 0.3). Chemotaxis by Kuppfer cells in response to f-met-leu-phe was also blunted by ethanol at 3 and 24 post-treatment. At 3 hr post-ethanol, the total number of binding sites and KDfor f-met-leu-phe on these cells were reduced by almost 30%. The concentration and KDof high affinity binding sites and chemotactic activity of Kupffer cells were not significantly altered by ethanol at 3 hr. However, by 24 hr these were profoundly depressed.
