BACTERIOPHAGE-PHI-X174-SPECIFIC MESSENGER-RNA SYNTHESIS IN CELLS DEFICIENT IN TERMINATION FACTOR-RHO ACTIVITY
BACTERIOPHAGE-PHI-X174-SPECIFIC MESSENGER-RNA SYNTHESIS IN CELLS DEFICIENT IN TERMINATION FACTOR-RHO ACTIVITY
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DOI：
10.1128/jvi.38.1.198-207.1981
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发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
IMAI, M
中科院分区：
文献类型：
--
作者：
HAYASHI, MN;HAYASHI, M;IMAI, M
先前的一份报告表明，体内的噬菌体φX174 mRNA在基因J、F、G和H之后终止。在这些位点的终止并不严格。为了确定φX174转录的终止是否取决于ρ因子的活性，将一个温度敏感的ρ突变（nitA）引入到一个对φX174敏感的宿主细胞（大肠杆菌）系中，并确定了野生型和nitA细胞中的终止位点。在感染了φX174的nitA细胞中识别出了正常的φX174终止子，ρ突变缓解了由噬菌体基因组中的无义突变或宿主细胞经氯霉素处理所导致的极性效应，并且极性不是由在极性突变位点处转录的过早终止所引起的。RNA合成继续超越该位点直至第一个对ρ敏感的位点。
A previous report indicated that in vivo bacteriophage .vphi.X174 mRNA terminate after genes J, F, G and H. Termination at these sites is not stringent. To determine whether termination of .vphi.X174 transcription depends on rho factor activity, a temperature-sensitive rho mutation (nitA) was introduced into a .vphi.X174-sensitive host cell (Escherichia coli) line and termination sites in wild-type and nitA cells were determined. Normal .vphi.X174 terminators were recognized in .vphi.X174-infected nitA cells, the rho mutation relieved polar effects caused by nonsense mutations in the phage genome or by chloramphenicol treatment of the host cells and polarity was not caused by premature termination of transcription at the site of the polar mutation. RNA synthesis continued beyond the site to the 1st rho-sensitive site.