Transglycosylation reaction of a chitinase purified from Nocardia orientalis.
Transglycosylation reaction of a chitinase purified from Nocardia orientalis.
复制标题
从东方诺卡氏菌中纯化的几丁质酶的转糖基反应。
DOI：
10.1016/0304-4165(87)90017-1
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Y. Ishido
中科院分区：
文献类型：
--
作者：
T. Usui;Y. Hayashi;F. Nanjo;K. Sakai;Y. Ishido
东方诺卡氏菌培养滤液经硫酸铵沉淀、CM-Sephadex和Bio-Gel P-60层析、苯基-SepharoseCL-4B亲和层析等步骤，可将几丁质酶纯化为表观均一的几丁质酶。该酶本质上是一种水解酶，它还催化四-N-乙酰基壳四糖(GlcNAc)4和五-N-乙酰基壳五糖(GlcNAc)5的转糖基化反应。该酶将四糖转化为六-N-乙酰基壳六糖(GlcNAc)6(21%)和二-N-乙酰基壳二糖(GlcNAc)2(63%)。五-N-乙酰基壳五糖(GlcNAc)5的对应值为七-N-乙酰基壳七糖(GlcNAc)723%，三-N-乙酰基壳三糖(GlcNAc)359%。转糖速率与温度、底物浓度和pH有关。
Chitinase from the culture filtrates ofNocardia orientalisIFO 12806 was purified to apparent homogeneity by precipitation with ammonium sulfate followed by successive chromatography on CM-Sephadex and Bio-Gel P-60, and finally by affinity chromatography on a phenyl-Sepharose CL-4B column. The enzyme, which is essentially a hydrolase, also catalyzed a transglycosylation reaction on tetra-N-acetyl-chitotetraose (GlcNAc)4and penta-N-acetyl-chitopentaose (GlcNAc)5. The enzyme converted the tetrasaccharide into hexa-N-acetyl-chitohexaose (GlcNAc)6(21%) and di-N-acetyl-chitobiose (GlcNAc)2(63%) as the major products. The corresponding values for penta-N-acetyl-chitopentaose (GlcNAc)5were hepta-N-acetyl-chitoheptaose (glcNAc)723% and tri-N-acetyl-chitotriose (GlcNAc)359%. The rate of the transglycosylation depended on the temperature, the concentration of substrate and the pH.