Identification of the activation-induced cytidine deaminase gene from zebrafish:: an evolutionary analysis
Identification of the activation-induced cytidine deaminase gene from zebrafish:: an evolutionary analysis
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DOI：
10.1016/j.dci.2004.05.005
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发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Hammarström, L
中科院分区：
文献类型：
--
作者：
Zhao, YF;Pan-Hammarström, Q;Hammarström, L
在本研究中，我们报告的激活诱导的胞苷脱氨酶（AID）编码基因在青蛙，狗和黑猩猩，其中体细胞超突变和类转换重组（CSR）的发生和斑马鱼和河豚，物种缺乏CSR的鉴定。本文报道的斑马鱼AID的cDNA序列表明先前预测的蛋白质序列的N和C端都是不正确的。在哺乳动物，鸟类，两栖动物和鱼类之间的AID序列的比较显示保守的氨基酸残基，这可能是必不可少的AID活性，虽然在后者的胞苷脱氨酶活性基序是9个氨基酸长。此外，来自硬骨鱼的AID的C末端中的aa缺失和广泛取代表明该分子可能尚未开发出募集启动CSR所需的特异性辅因子的能力。(C)2004爱思唯尔有限公司保留所有权利。
In the present study, we report the identification of the activation-induced cytidine deaminase (AID) encoding gene in frog, dog and chimpanzee, where both somatic hypermutation and class switch recombination (CSR) occurs and in zebrafish and fugu, species lacking CSR. The cDNA sequence of the zebrafish AID reported here suggests both N and C ends of the previously predicted protein sequence are incorrect. A comparison of AID sequences among mammals, birds, amphibians and fish revealed conserved aa residues which may be essential for AID activity, although the cytidine deaminase active motif in the latter is nine amino acids longer. Furthermore, an aa deletion, and extensive substitutions in the C terminal end of AID from bony fish indicate that the molecule may not yet have developed a capacity to recruit the specific cofactor(s) needed to initiate CSR. (C) 2004 Elsevier Ltd. All rights reserved.