Haplogroup relationships between domestic and wild sheep resolved using a mitogenome panel
Haplogroup relationships between domestic and wild sheep resolved using a mitogenome panel
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DOI：
10.1038/hdy.2010.122
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发表时间：
2011-04-01
期刊：
影响因子：
3.8
通讯作者：
Kijas, J. W.
中科院分区：
文献类型：
--
作者：
Meadows, J. R. S.;Hiendleder, S.;Kijas, J. W.
通过对线粒体（mt）序列变异的全球调查，已经在家养绵羊中确定了五个单倍型群，然而这些群分类通常基于完整mtDNA序列的小片段、部分控制区或细胞色素B基因。这项研究提出了完整的有丝分裂基因组的代表，每个单倍型群确定在国内绵羊，加上他们的野生亲戚的样品。序列的比较成功地解决了每个单倍型群之间的关系，并提供了深入了解与野生绵羊的关系。这五个单倍群的特征是独立分支，根据蛋白质编码序列，它们在920 000 +/- 190 000年前有一个共同的祖先。利用不同的mtDNA成分，告知羊之间的真实关系，也检查贝叶斯，最大似然和分区Bremmer支持分析。控制区被认为是mtDNA的组成部分，这有助于最高量的支持使用完整的数据集生成的树。这项研究提供了一个mtDNA有丝分裂基因组组面板，它可以用来评估额外的有丝分裂基因组的核心，并作为一个参考集，以评估小片段的mtDNA。Heredity（2011）106，700-706; doi：10.1038/hdy.2010.122; 2010年10月13日在线发表
Five haplogroups have been identified in domestic sheep through global surveys of mitochondrial (mt) sequence variation, however these group classifications are often based on small fragments of the complete mtDNA sequence; partial control region or the cytochrome B gene. This study presents the complete mitogenome from representatives of each haplogroup identified in domestic sheep, plus a sample of their wild relatives. Comparison of the sequence successfully resolved the relationships between each haplogroup and provided insight into the relationship with wild sheep. The five haplogroups were characterised as branching independently, a radiation that shared a common ancestor 920 000 +/- 190 000 years ago based on protein coding sequence. The utility of various mtDNA components to inform the true relationship between sheep was also examined with Bayesian, maximum likelihood and partitioned Bremmer support analyses. The control region was found to be the mtDNA component, which contributed the highest amount of support to the tree generated using the complete data set. This study provides the nucleus of a mtDNA mitogenome panel, which can be used to assess additional mitogenomes and serve as a reference set to evaluate small fragments of the mtDNA. Heredity (2011) 106, 700-706; doi:10.1038/hdy.2010.122; published online 13 October 2010