ASSOCIATION OF PROTEIN AMOUNT POLYMORPHISM (PAP) AMONG MAIZE LINES WITH PERFORMANCES OF THEIR HYBRIDS
ASSOCIATION OF PROTEIN AMOUNT POLYMORPHISM (PAP) AMONG MAIZE LINES WITH PERFORMANCES OF THEIR HYBRIDS
复制标题
DOI：
10.1007/bf00226790
复制
发表时间：
1991-01-01
影响因子：
5.4
通讯作者：
DEVIENNE, D
中科院分区：
文献类型：
--
作者：
LEONARDI, A;DAMERVAL, C;DEVIENNE, D
已经表明，表型多样性的分子基础在于基因组表达的变异性。这种变异性可以通过单个蛋白质量的多态性（PAP：蛋白质量多态性）来评估。为探讨PAP在预测杂种优势中的应用价值，采用双向聚丙烯酰胺凝胶电泳技术对8个玉米自交系及其10个单交种进行了分析。28对可能的品系的特征在于：（i）在2D-PAGE显示的基因座样品中，其杂种中预期杂合结构基因座的数目H;（ii）基于PAP的四个距离指数;（iii）在四个不同年份/地点测量的五个农业形态性状的杂种值。对于通过2D-PAGE分析的10个杂种的子集，还计算了非加性遗传（NA）的病例数。而H似乎既不与PAP指数，也不NA，也不混合性能，PAP指数与NA相关，两者均与混合性能呈正相关。讨论了PAP对数量性状变异的可能性。这可能导致杂种优势的生物预测因子的定义。
It has been suggested that molecular foundations of phenotypic diversity reside in the variability of genome expression. This variability can be appraised through the polymorphism of individual protein amounts (PAP: protein amount polymorphism). Eight maize inbred lines and ten of their single-cross hybrids were analyzed by two-dimensional polyacrylamide gel electrophoresis in order to examine the potential of PAP for predicting hybrid vigor. The 28 possible pairs of lines were characterized for: (i) the number, H of expected heterozygous structural loci in their hybrid, in the sample of loci revealed by 2D-PAGE; (ii) four distance indices based on PAP; (iii) the hybrid values for five agromorphological characters measured in four different year/locations. For the subset of ten hybrids analyzed by 2D-PAGE, the number of cases of nonadditive inheritance (NA) was also counted. Whereas H appeared to be related neither to the PAP indices, nor to NA, nor to hybrid performances, PAP indices were correlated to NA, and both were positively associated to hybrid performances. The possibility that PAP is responsible for quantitative trait variation is discussed. This could result in the definition of biological predictors of heterosis.