Silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligament
Silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligament
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DOI：
10.1186/s12891-019-2777-8
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发表时间：
2019-08-31
影响因子：
2.3
通讯作者：
Tanaka, Yasuhito
中科院分区：
文献类型：
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作者：
Egawa, Takuya;Inagaki, Yusuke;Tanaka, Yasuhito
背景前交叉韧带损伤的治疗通常涉及使用聚对苯二甲酸乙二醇酯（PET）人工韧带进行重建。然而，目前可用的方法需要较长的固定时间，从而需要开发替代方法来加速肌腱和骨骼之间的愈合过程。因此，我们开发和评估了一种新的技术，利用硅酸盐取代锶（SrSiP）。方法制备SrSiP纳米涂层PET薄膜。培养雄性大鼠股骨的骨髓间充质细胞（BMSC），并以1.0 × 10（4）/cm（2）的密度接种到SrSiP涂层和非涂层PET膜上，随后置于成骨培养基中。在每种情况下，比较分泌到培养基中的骨钙素浓度。然后，制备了纳米SrSiP涂层的PET人工韧带。将BMSC以4.5 × 10（5）/cm（2）的密度接种到SrSiP涂层和非涂层人工韧带上，然后置于成骨培养基中。在培养的第8、10、12和14天测量培养基中的骨钙素和钙浓度。此外，通过qPCR评估了骨钙素、碱性磷酸酶（ALP）、骨形态发生蛋白-2（BMP 2）和侏儒相关转录因子2（Runx 2）的mRNA表达。我们将SrSiP涂层和未涂层人工韧带移植到成年新西兰白色兔胫骨上。两个月后，我们处死它们并进行组织学评估。结果SrSiP组膜上培养液中骨钙素分泌量明显高于未包被组。在第14天，SrSiP组中人工韧带上培养基中的分泌性骨钙素浓度也显著高于未涂覆组。在第8、10、12和14天，SrSiP组中人工韧带上的钙浓度显著低于无涂层组。同样在qPCR中，SrSiP组的OC、ALP、BMP 2和Runx 2 mRNA表达显著高于未涂覆组。SrSiP组人工韧带周围组织学上可见新骨形成。结论SrSiP人工韧带具有良好的成骨能力，有望在临床上得到应用。
Background Treatment of anterior cruciate ligament injuries commonly involves the use of polyethylene terephthalate (PET) artificial ligaments for reconstruction. However, the currently available methods require long fixation periods, thereby necessitating the development of alternative methods to accelerate the healing process between tendons and bones. Thus, we developed and evaluated a novel technique that utilizes silicate-substituted strontium (SrSiP). Methods PET films, nano-coated with SrSiP, were prepared. Bone marrow mesenchymal cells (BMSCs) from femurs of male rats were cultured and seeded at a density of 1.0 x 10(4)/cm(2) onto the SrSiP-coated and non-coated PET film, and subsequently placed in an osteogenic medium. The osteocalcin concentration secreted into the medium was compared in each case. Next, PET artificial ligament, nano-coated with SrSiP, were prepared. BMSCs were seeded at a density of 4.5 x 10(5)/cm(2) onto the SrSiP-coated, and non-coated artificial ligament, and then placed in osteogenic medium. The osteocalcin and calcium concentrations in the culture medium were measured on the 8th, 10th, 12th, and 14th day of culture. Furthermore, mRNA expression of osteocalcin, alkaline phosphatase (ALP), bone morphogenetic protein-2 (BMP2), and runt-related transcription factor 2 (Runx2) was evaluated by qPCR. We transplanted the SrSiP-coated and non-coated artificial ligament to the tibiae of mature New Zealand white rabbits. Two months later, we sacrificed them and histologically evaluated them. Results The secretory osteocalcin concentration in the medium on the film was significantly higher for the SrSiP group than for the non-coated group. Secretory osteocalcin concentration in the medium on the artificial ligament was also significantly higher in the SrSiP group than in the non-coated group on the 14th day. Calcium concentration on the artificial ligament was significantly lower in the SrSiP group than in the non-coated group on the 8th, 10th, 12th, and 14th day. In qPCR as well, OC, ALP, BMP2, and Runx2 mRNA expression were significantly higher in the SrSiP group than in the non-coated group. Newly formed bone was histologically found around the artificial ligament in the SrSiP group. Conclusions Our findings demonstrate that artificial ligaments using SrSiP display high osteogenic potential and thus may be efficiently used in future clinical applications.