Diversity of low-molecular-weight glutenin subunit genes in Asian common wheat (Triticum aestivum L.)
Diversity of low-molecular-weight glutenin subunit genes in Asian common wheat (Triticum aestivum L.)
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DOI：
10.1270/jsbbs.55.349
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发表时间：
2005-09-01
期刊：
影响因子：
2.4
通讯作者：
Tsujimoto, H
中科院分区：
文献类型：
--
作者：
Tanaka, H;Toyoda, S;Tsujimoto, H
分析了亚洲小麦品种低分子量麦谷蛋白亚基（LMW-GS）基因的多样性。基因座特异性引物的LMW-GS揭示了存在四个等位基因在Glu-A3位点，四个在Glu-B3位点和一个在Glu-D3位点。日本品种的Glu-A3等位基因频率与其他亚洲品种有很大差异。3个Glu-3基因座的等位基因可分为15种基因型。来自亚洲收集或繁殖区的每种基因型的频率各不相同。Glu-3基因型BAA（由Glu-A3等位基因B、Glu-B3等位基因A和Glu-D3等位基因A组成）在许多亚洲品种中频繁出现。Glu-3基因型BBA、CAA和DBA分别在南亚、北方和日本占优势。此外，日本品种与BBA或CAA基因型表现出特定的高分子量麦谷蛋白亚基和醇溶蛋白的组成。DNA测序表明，Glu-A3等位基因B携带一个额外的半胱氨酸残基，等位基因C显示不寻常的氨基酸缺失。这些突变可能会影响面团强度，这是小麦面粉品质的主要特征。
We analyzed the diversity of the low-molecular-weight glutenin subunit (LMW-GS) genes of Asian common wheat cultivars. Locus-specific primers for LMW-GSs revealed the presence of four alleles at the Glu-A3 locus, four at the Glu-B3 locus and one at the Glu-D3 locus. Frequency of Glu-A3 alleles in the Japanese cultivars was very different from that in other Asian cultivars. The combined alleles at the three Glu-3 loci were classified into 15 genotypes. The frequency of each genotype from the Asian collection or breeding areas varied. One Glu-3 genotype, BAA (consisting of the Glu-A3 allele B, Glu-B3 allele A and Glu-D3 allele A), occurred frequently in many Asian cultivars. Glu-3 genotypes BBA, CAA and DBA predominated in southern and northern Asia and in Japan, respectively. In addition, the Japanese cultivars with the BBA or CAA genotypes showed a specific composition of high-molecular-weight glutenin subunits and gliadins. DNA sequencing indicated that the Glu-A3 allele B carried an additional cysteine residue and that the allele C showed unusual amino-acid deletions. These mutations were likely to affect dough strength, which is a major characteristic of wheat flour quality.