INDUCTION OF PROTECTIVE IMMUNITY AGAINST RABIES BY IMMUNIZATION WITH RABIES VIRUS RIBONUCLEOPROTEIN
INDUCTION OF PROTECTIVE IMMUNITY AGAINST RABIES BY IMMUNIZATION WITH RABIES VIRUS RIBONUCLEOPROTEIN
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DOI：
10.1073/pnas.84.24.9165
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发表时间：
1987-12-01
影响因子：
11.1
通讯作者：
KOPROWSKI, H
中科院分区：
文献类型：
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作者：
DIETZSCHOLD, B;WANG, HH;KOPROWSKI, H
我们研究了狂犬病病毒核糖核蛋白（RNP）诱导动物对狂犬病病毒和狂犬病相关狂犬病病毒致死性攻击的保护性免疫应答的能力。脂质体含有RNP或糖蛋白（G蛋白）的变异病毒与多重改变G抗原结构赋予没有或差的保护，分别对致命的脑内狂犬病病毒的挑战。相比之下，含有RNP和变体G蛋白的脂质体诱导了良好的保护性应答，与针对脑内攻击的灭活病毒疫苗所实现的保护性应答相当。此外，单独用RNP免疫的小鼠或浣熊抵抗同源或异源病毒株的致死性外周攻击。这些结果表明，狂犬病病毒的RNP在诱导保护性免疫中起着至关重要的作用。
We have studied the ability of rabies virus ribonucleoprotein (RNP) to induce a protective immune response in animals against lethal challenge with rabies and rabies-related lyssa viruses. Liposomes containing either RNP or the glycoprotein (G protein) of a variant virus with multiple alterations in the G antigenic structure conferred no or poor protection, respectively, against lethal intracerebral challenge with rabies virus. By contrast, liposomes containing RNP and the variant G protein induced a good protective response, comparable to that achieved with inactivated virus vaccine against intracerebral challenge. Moreover, mice or raccoons immunized with RNP alone resisted lethal peripheral challenge with homologous or heterologous virus strains. These results indicate that the RNP of rabies virus plays a crucial role in induction of protective immunity.