A novel role of luteinizing hormone in the embryo development in cocultures.
A novel role of luteinizing hormone in the embryo development in cocultures.
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黄体生成素在共培养胚胎发育中的新作用。
DOI：
10.1095/biolreprod.102.011874
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Rao,ChV
中科院分区：
文献类型：
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作者：
Mishra,S;Lei,ZM;Rao,ChV
牛输卵管上皮含有LH受体，其功能是促进输卵管糖蛋白（OGP）的合成。与胚胎与输卵管上皮细胞的共培养一样，OGP被认为促进早期胚胎的生长和发育。这些发现使我们验证了LH处理共培养物通过OGP介导进一步增加胚胎发育的假设。将≥10枚牛二细胞胚胎与牛输卵管上皮细胞共培养，可提高胚胎的囊胚发育率。用hCG处理这些共培养物，由于其稳定性和纯度而用作LH的替代物，进一步增加了胚胎发育。hCG的作用是剂量依赖性和激素特异性的，需要二聚体构象和输卵管上皮细胞中LH受体的存在。抑制OGP的合成和阻止蛋白激酶A的激活阻断了共培养物中hCG的作用。逆转录聚合酶链反应和间接免疫荧光与激光扫描共聚焦显微镜证明存在LH受体在牛卵母细胞，胚胎和囊胚。然而，胚胎LH受体可能在共培养中的有益hCG效应中没有发挥任何作用。这些发现表明排卵期LH水平升高可能促进输卵管着床前胚胎发育。这些结果对辅助生殖技术具有重要意义，其中共培养用于提高妊娠率。
Bovine oviductal epithelium contains LH receptors, which function in the increase of synthesis of oviductal glycoprotein (OGP). As with cocultures of embryos with oviductal epithelial cells, OGP is thought to promote early embryonic growth and development. These findings led us to test the hypothesis that LH treatment of cocultures further increases embryo development through OGP mediation. Coculture of ≥10 two-cell bovine embryos with bovine oviductal epithelial cells increased the development of the embryos into blastocysts. Treatment of these cocultures with hCG, used as a surrogate for LH because of its stability and purity, further increased embryo development. The hCG effect is dose dependent and hormone specific and requires the dimer conformation and the presence of LH receptors in oviductal epithelial cells. The inhibition of OGP synthesis and prevention of protein kinase A activation blocked the hCG effect in cocultures. Reverse transcription polymerase chain reaction and indirect immunofluorescence with laser scanning confocal microscopy demonstrated the presence of LH receptors in bovine oocytes, embryos, and blastocysts. However, embryo LH receptors may not have played any role in the beneficial hCG effects in cocultures. These findings suggest that elevated periovulatory LH levels may promote preimplantation embryo development in oviducts. These results have important implications for assisted reproductive technologies in which cocultures are used to improve pregnancy rates.