Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea
Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea
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DOI：
10.3906/sag-1510-94
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发表时间：
2017-01-01
影响因子：
2.3
通讯作者：
Ozkan, Secil
中科院分区：
文献类型：
--
作者：
Colak, Meryem;Bozdayi, Gulendam;Ozkan, Secil
背景/目的：为了确定的频率，基因型分布，腺病毒在5岁以下的儿童腹泻和遗传相关性，并调查其分布根据临床表现，年龄，月份和seasons.Materials和方法：粪便样本收集了180名儿童急性胃肠炎谁从2007年7月至2011年6月在安卡拉培训和教育医院。通过免疫层析试验（ICT）、酶免疫测定（EIA）和聚合酶链反应（PCR）分析粪便样本。通过核苷酸序列分析确定所有腺病毒类型。结果：180份标本中，ICT检测腺病毒阳性率为5%（9/180），EIA检测腺病毒阳性率为6.1%（11/180），PCR检测腺病毒阳性率为13.9%（25/180）。腺病毒性胃肠炎在年龄组、性别、月份或季节中没有表现出任何差异。PCR阳性标本经核苷酸序列测定，AdV 41 16例（64%），AdV 40 6例（24%），AdV 31 2例（8%），AdV 7 1例（4%）。腺病毒血清型与亚洲和美洲血清型的相似性分别为80%（20/25）和20%（5/25）。
Background/aim: To determine the frequency, genotype distribution, and genetic relatedness of adenoviruses in children under 5 years old with diarrhoea and to investigate their distribution according to clinical findings, age, months, and seasons.Materials and methods: Stool samples were collected from 180 children with acute gastroenteritis who presented from July 2007 through June 2011 at the Ankara Training and Education Hospital. Stool samples were analysed by immune chromatographic test (ICT), enzyme immunoassay (EIA), and polymerase chain reaction (PCR). All adenovirus types were determined by nucleotide sequence analysis. A phylogenetic tree was constructed by Mega 6.0 using the neighbour-joining method.Results: Five percent of the samples were positive for adenovirus (9/180) by ICT, 6.1% (11/180) by EIA, and 13.9% (25/180) by PCR. Adenovirus gastroenteritis did not show any differences in age group, sex, month, or season. In this study, 16 (64%) of the PCR positive samples were AdV41, 6 (24%) were AdV40, 2 (8%) were AdV31, and 1 (4%) was AdV7, as determined by nucleotide sequencing.Conclusion: AdV31 and AdV7 were associated with gastroenteritis. Adenovirus serotypes showed a similarity of 80% (20/25) and 20% (5/25) with Asian and American serotypes, respectively.