Alterations of CCR2 and CX3CR1 on Three Monocyte Subsets During HIV-1/Treponema pallidumCoinfection
Alterations of CCR2 and CX3CR1 on Three Monocyte Subsets During HIV-1/Treponema pallidumCoinfection
复制标题
DOI：
10.3389/fmed.2020.00272
复制
发表时间：
2020-06-18
影响因子：
3.9
通讯作者：
Zhang, Tong
中科院分区：
文献类型：
--
作者：
Guo, Na;Chen, Yongchang;Zhang, Tong
HIV-1/梅毒螺旋体混合感染已成为一个全球性的挑战，三个单核细胞亚群表达不同水平的趋化因子受体CCR2和CX3CR1。我们最近评估了HIV感染梅毒患者的单核细胞亚群和调节性T细胞之间的关系。目前，单核细胞亚群CCR2和CX3CR1在HIV-1和梅毒混合感染过程中的动态变化还没有得到充分的研究。本研究采用细胞表面染色技术检测了HIV-1/梅毒螺旋体感染过程中CCR2和CX3CR1在三个单核细胞亚群上的表达。我们发现，在急性HIV-1感染(AHI)患者、未接受抗病毒治疗(CHI+ART-)的慢性HIV-1感染者(CHI+ART-)、接受ART治疗的慢性HIV-1感染者(CHI+ART+)、快速血浆反应素阳性(RPR+)患者、CHI+ART+RPR+(CHI+RPR+ART-)患者以及CHI+ART++RPR+(CHI+RPR+ART+)患者中，经典单核细胞亚群CCR2密度降低。在HIV-1和/或ORT期间，CX3CR1在三个单核细胞亚群上的密度增加。梅毒螺旋体感染。CHI+ART+组CX3CR1密度低于CHI+ART+组，CHI+ART+组CX3CR1密度高于CHI+RPR+ART+组。我们的数据为CCR2和CX3CR1在HIV-1和T细胞中的三个单核细胞亚群上的作用提供了新的见解。苍白球的发病机制。
HIV-1/Treponema pallidum(T. pallidum) coinfection has become a global challenge, and three monocyte subsets express varying levels of the chemokine receptors CCR2 and CX3CR1. We recently evaluated the association between monocyte subsets and regulatory T cells in HIV-infected individuals with syphilis. Currently, the dynamic changes of CCR2 and CX3CR1 on monocyte subsets during HIV-1 and syphilis coinfection have not been fully investigated. In this study, cell surface staining was used to explore CCR2 and CX3CR1 expression on three monocyte subsets during HIV-1/T. pallidumcoinfection. We found that CCR2 densities on the classical monocyte subsets decreased in acute HIV-1 infected (AHI) patients, chronic HIV-1-infected individuals without antiviral therapy (ART) (CHI+ ART-), chronic HIV-1-infected individuals receiving ART (CHI+ART+), rapid plasma reagin-positive (RPR+) individuals, CHI+ ART- plus RPR+ (CHI+RPR+ ART-) individuals, and CHI+ART+ plus RPR+(CHI+RPR+ART+) individuals. CX3CR1 density increased on the three monocyte subsets during HIV-1 and/orT. palliduminfection. CX3CR1 density on the intermediate and non-classical monocyte subsets in CHI+ ART- individuals was lower than that in CHI+ART+ individuals, and CX3CR1 density on the three monocyte subsets in CHI+ART+ individuals was higher than that in CHI+RPR+ART+ individuals. Our data provide new insight into the roles of CCR2 and CX3CR1 on three monocyte subsets in HIV-1 andT. pallidumpathogenesis.