Cartilage fragments from osteoarthritic knee promote chondrogenesis of mesenchymal stem cells without exogenous growth factor induction
Cartilage fragments from osteoarthritic knee promote chondrogenesis of mesenchymal stem cells without exogenous growth factor induction
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DOI：
10.1002/jor.21541
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发表时间：
2012-03-01
影响因子：
2.8
通讯作者：
Fang, Hsu-Wei
中科院分区：
文献类型：
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作者：
Chen, Chia-Chun;Liao, Cheng-Hao;Fang, Hsu-Wei
细胞外基质（ECM）被认为在引导间充质干细胞（MSCs）分化过程中起重要作用。在这项研究中，我们假设软骨碎片从骨关节炎膝关节可以促进骨髓间充质干细胞的软骨形成。在全膝关节置换术（TKA）过程中获得软骨组织的未磨损部分。将软骨碎片和MSC包裹在纤维蛋白胶中;并将构建体皮下植入裸鼠体内。组织学分析显示，在软骨碎片纤维蛋白MSC构建体中，新软骨样结构具有阳性阿辛蓝染色。然而，在纤维蛋白中仅具有MSC的构建体显示出与沉淀培养物中的MSC一样的浓缩外观。植入4周后，含60 mg软骨碎片的构建体中II型胶原的基因表达显著升高。相反，没有软骨碎片的构建体不能表达II型胶原，这表明MSC没有分化成软骨形成谱系。总之，我们证明了骨关节炎膝关节软骨碎片在促进骨髓间充质干细胞软骨分化中的作用。这可能是一个有利的策略，MSC软骨细胞没有外源性生长因子诱导。(C)2011年由Wiley Periodicals，Inc.出版的骨科研究学会。J Orthop Res 30：393400，2012
Extracellular matrix (ECM) is thought to participate significantly in guiding the differentiation process of mesenchymal stem cells (MSCs). In this study, we hypothesized that cartilage fragments from osteoarthritic knee could promote chondrogenesis of MSCs. Nonworn parts of cartilage tissues were obtained during total knee arthroplasty (TKA) surgery. Cartilage fragments and MSCs were wrapped into fibrin glue; and the constructs were implanted subcutaneously into nude mice. Histological analysis showed neocartilage-like structure with positive Alcian blue staining in the cartilage fragmentfibrinMSC constructs. However, constructs with only MSCs in fibrin showed condensed appearance like MSCs in the pellet culture. Gene expression of type II collagen in the constructs with 60 mg cartilage fragments were significantly elevated after 4 weeks of implantation. Conversely, the constructs without cartilage fragments failed to express type II collagen, which indicated MSCs did not differentiate into a chondrogenic lineage. In conclusion, we demonstrated the effect of cartilage fragments from osteoarthritic knee in promoting chondrogenic differentiation of MSCs. This may be a favorable strategy for MSC chondrogenesis without exogenous growth factor induction. (C) 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:393400, 2012