Purification and Characterization of Plantaricin 163, a Novel Bacteriocin Produced by Lactobacillus plantarum 163 Isolated from Traditional Chinese Fermented Vegetables
Purification and Characterization of Plantaricin 163, a Novel Bacteriocin Produced by Lactobacillus plantarum 163 Isolated from Traditional Chinese Fermented Vegetables
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DOI：
10.1021/jf403370y
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发表时间：
2013-11-27
影响因子：
6.1
通讯作者：
Lu, Zhaoxin
中科院分区：
文献类型：
--
作者：
Hu, Meizhong;Zhao, Haizhen;Lu, Zhaoxin
对从中国传统发酵蔬菜中分离出的假定乳酸菌（LAB）菌株进行细菌素生产筛选。根据其生理生化特征并通过 16S rDNA 测序鉴定了一种新的细菌素生产菌株植物乳杆菌 163。新型细菌素 Plantaricin 163，由 Lb. 生产。 plantarum 163 通过盐沉淀、凝胶过滤和反相高效液相色谱 (RP-HPLC) 进行纯化。 Plantaricin 163 的基质辅助激光解吸/电离飞行时间质谱 (MALDI-TOF-MS) 分析显示其分子量为 3553.2 Da。完整的氨基酸序列显示为VFHAYSARGNYYGNCPANVVPSCRNNYKSAGGK，未发现与已知细菌素的相似性。 Plantaricin 163 具有高度热稳定性（20 分钟，121 摄氏度），在酸性 pH 值 (3-5) 下具有活性，对蛋白酶敏感，并且对 LAB 和其他测试的革兰氏阳性和革兰氏阴性细菌表现出广谱抗菌活性。结果表明 Plantaricin 163 可用作食品工业中的生物防腐剂。
Presumptive lactic acid bacteria (LAB) strains isolated from traditional Chinese fermented vegetables were screened for bacteriocin production. A novel bacteriocin-producing strain, Lactobacillus plantarum 163, was identified on the basis of its physiobiochemical characteristics and characterized by 16S rDNA sequencing. The novel bacteriocin, plantaricin 163, produced by Lb. plantarum 163 was purified by salt precipitation, gel filtration, and reverse-phase high-performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of plantaricin 163 revealed the molecular weight to be 3553.2 Da. The complete amino acid sequence showed VFHAYSARGNYYGNCPANVVPSCRNNYKSAGGK, and no similarity to known bacteriocins was found. Plantaricin 163 was highly thermostable (20 min, 121 degrees C), active in the presence of acidic pH (3-5), sensitive to protease, and exhibited broad-spectrum antimicrobial activity against LAB and other tested Gram-positive and Gram-negative bacteria. The results suggest that plantaricin 163 may be employed as a biopreservative in the food industry.