Ferritin-Enhanced Direct MicroRNA Detection via Controlled Radical Polymerization.
Ferritin-Enhanced Direct MicroRNA Detection via Controlled Radical Polymerization.
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DOI：
10.1021/acs.analchem.2c04063
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发表时间：
2022-12
影响因子：
7.4
通讯作者：
N. Ma;Yu Zhao;Lianzhi Li;J. Kong;Xueji Zhang
中科院分区：
文献类型：
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作者：
N. Ma;Yu Zhao;Lianzhi Li;J. Kong;Xueji Zhang
准确定量检测示踪核酸仍然是癌症基因检测中的一个巨大挑战。因此，提出一种低成本、高灵敏度的直接基因检测方法对于癌症的预防和治疗至关重要。在此，这项工作报告了一个超灵敏的生物传感器通过铁蛋白增强原子转移自由基聚合（Ft-ATRP）过程。有趣的是，microRNA-21，肺癌的早期标志物，可以通过一种创新的信号放大策略，使用铁蛋白介导的亲水性氮氧自由基单体的聚集作为电化学生物传感器来检测，而无需预先转录。该传感器使用修饰在金电极上的肽核酸探针，准确结合样本中的目标肺癌标志物，然后在温和条件下，人体血液中天然存在的铁蛋白在电极表面诱导Ft-ATRP。许多具有电化学信号的4-甲基丙烯酰氧基-2，2，6，6-四甲基哌啶1-氧自由基（MATMP）单体被结合成聚合物链以用于靶向测定。microRNA-21的检测限（LOD）低至6.03 fM，检测浓度范围为0.01 - 100 pM（R2 = 0.994）。本发明的RNA生物传感器能够以简单的操作实现对复杂样品的高性能分析，而且所使用的催化剂、含有电化学信号的聚合物和电解质溶液的检测过程都具有良好的水溶性。RNA生物传感器的上级性能证明了其在目标患者中筛查和识别肺癌的潜力。
Accurate quantitative detection of tracing nucleic acids remains a great challenge in cancer genetic testing. It is crucial to propose a low-cost and highly sensitive direct gene detection method for cancer prevention and treatment. Herein, this work reports an ultrasensitive biosensor via a ferritin-enhanced atom-transfer radical polymerization (Ft-ATRP) process. Intriguingly, microRNA-21, an early marker of lung cancer, can be detected without being transcribed in advance by an innovative signal amplification strategy using ferritin-mediated aggregation of hydrophilic nitroxide radical monomers as an electrochemical biosensor. The sensor uses peptide nucleic acid probes modified on a gold electrode to accurately bind the target lung cancer marker in the sample, and then ferritin, which is naturally present in human blood, induces Ft-ATRP on the electrode surface under mild conditions. Many of 4-methacryloyloxy-2,2,6,6-tetramethylpiperidine 1-oxyl free radical (MATMP) monomers with electrochemical signals are combined into polymeric chains to be modified on target assays. The limit of detection (LOD) of microRNA-21 is as low as 6.03 fM, and the detection concentration ranges from 0.01 to 100 pM (R2 = 0.994). The RNA biosensor can realize great performance analysis of complicated samples in simple operation, in addition, the detection process used by the catalyst, polymers containing electrochemical signals, and the electrolyte solution all have good water solubility. The superior performance of the RNA biosensor demonstrates its potential to screen and identify lung cancer in target patients.