Comprehensive gene and microRNA expression profiling on cardiovascular system in zebrafish co-exposured of SiNPs and MeHg
Comprehensive gene and microRNA expression profiling on cardiovascular system in zebrafish co-exposured of SiNPs and MeHg
复制标题
SiNPs 和 MeHg 共同暴露的斑马鱼心血管系统的综合基因和 microRNA 表达谱
DOI：
10.1016/j.scitotenv.2017.07.036
复制
发表时间：
2017-12-31
影响因子：
9.8
通讯作者：
Sun, Zhiwei
中科院分区：
文献类型：
--
作者：
Hu, Hejing;Shi, Yanfeng;Sun, Zhiwei
空气污染已被证明会增加心血管疾病。然而，很少有人关注PM和空气污染物对心血管系统的综合影响。为了探索这一点，高通量测序技术被用来确定二氧化硅纳米粒子（SiNPs）和甲基汞在斑马鱼的综合影响。我们的研究表明，SiNPs和甲基汞共同暴露可以引起斑马鱼的mRNA和miRNA表达模式的显着变化。STC分析的17和26号谱中的差异表达（DE）基因表明，SiNPs和甲基汞联合暴露比单独暴露对斑马鱼具有更大的促炎和心血管毒性。从动态基因网络图中识别出与共暴露斑马鱼心血管系统相关的主要基因功能，包括stxbp 1a、celf 4、ahr 1b和bai 2。此外，通过miRNA-pathway-network鉴定的3个DE miRNAs（dre-miR-7147，dre-miR-26 a和dre-miR-375）靶向心肌收缩的显著表达途径，其中包括23个DE基因。本研究从整体上揭示了SiNPs和甲基汞联合毒性对斑马鱼mRNA和miRNA动态表达的影响，为进一步研究SiNPs和甲基汞对斑马鱼心血管系统的毒性作用提供了基础研究线索。(C)2017爱思唯尔B. V.保留所有权利。
Air pollution has been shown to increase cardiovascular diseases. However, little attention has been paid to the combined effects of PM and air pollutants on the cardiovascular system. To explore this, a high-throughput sequencing technology was used to determine combined effects of silica nanoparticles (SiNPs) and MeHg in zebrafish. Our study demonstrated that SiNPs and MeHg co-exposure could cause significant changes in mRNA and miRNA expression patterns in zebrafish. The differentially expressed (DE) genes in profiles 17 and 26 of STC analysis suggest that SiNPs and MeHg co-exposure had more proinflammatory and cardiovascular toxicity in zebrafish than single exposure. Major gene functions associated with cardiovascular system in the co-exposed zebrafish were discerned from the dynamic-gene-network, including stxbp1a, celf4, ahr1b and bai2. In addition, the prominently expressed pathway of cardiac muscle contraction was targeted by 3 DE miRNAs identified by the miRNA-pathway-network (dre-miR-7147, dre-miR-26a and dre-miR-375), which included 23 DE genes. This study presents a global view of the combined SiNPs and MeHg toxicity on the dynamic expression of both mRNAs and miRNAs in zebrafish, and could serve as fundamental research clues for future studies, especially on cardiovascular system toxicity. (C) 2017 Elsevier B.V. All rights reserved.