GPCR annotation, G proteins, and transcriptomics of fire ant (Solenopsis invicta) queen and worker brain: An improved view of signaling in an invasive superorganism
GPCR annotation, G proteins, and transcriptomics of fire ant (Solenopsis invicta) queen and worker brain: An improved view of signaling in an invasive superorganism
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DOI：
10.1016/j.ygcen.2018.12.008
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发表时间：
2019-07-01
影响因子：
2.7
通讯作者：
Pietrantonio, Patricia, V
中科院分区：
文献类型：
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作者：
Calkins, Travis L.;Tamborindeguy, Cecilia;Pietrantonio, Patricia, V
G蛋白偶联受体（gpcr）及其信号传导模式的知识对于推进昆虫内分泌学至关重要，特别是在高度成功的入侵社会性昆虫中，如红进口火蚁（Solenopsis invicta Buren）。在首次发表的S. invicta基因组草图中，重点放在嗅觉受体的注释上，只报道了预测GPCR基因的数量。如果没有组织和管理的GPCRs资源，将很难测试关于神经肽激素内分泌作用的假设，或者神经递质和神经调节剂的功能。因此，我们挖掘了invicta基因组的gpcr，发现了由125个预测位点编码的324个预测转录本，并改进了其中55个位点的注释。其中有16个gpcr目前被标注为“未表征蛋白”。此外，本文提出的A类神经肽受体的系统发育分析，以及膜翅目昆虫、蜜蜂（均为群居）、玻璃翅目蜜蜂（独居）和独居模式双翅目果蝇（Drosophila melanogaster）中gpcr的比较清单，将有助于与社会昆虫进化和多样性相关的比较内分泌学研究。我们编译了从12个G蛋白基因（5 α， 5 β和2 γ）预测的24个G蛋白转录本（15 α， 7 β和2 γ）。该蚁种的生殖分工是极端的，因此，我们比较了工蚁、交配蚁后和未交配蚁后脑转录组中GPCR和G蛋白基因的表达。10个gpcr和2个G蛋白的转录本在蚁后和工蜂大脑中有差异表达。差异表达的gpcr是探索该物种劳动分工假说的候选受体。
Knowledge of G protein-coupled receptors (GPCRs) and their signaling modalities is crucial to advancing insect endocrinology, specifically in highly successful invasive social insects, such as the red imported fire ant, Solenopsis invicta Buren. In the first published draft genome of S. invicta, emphasis was placed on the annotation of olfactory receptors, and only the number of predicted GPCR genes was reported. Without an organized and curated resource for GPCRs, it will be difficult to test hypotheses on the endocrine role of neuropeptide hormones, or the function of neurotransmitters and neuromodulators. Therefore, we mined the S. invicta genome for GPCRs and found 324 predicted transcripts encoded by 125 predicted loci and improved the annotation of 55 of these loci. Among them are sixteen GPCRs that are currently annotated as "uncharacterized proteins". Further, the phylogenetic analysis of class A neuropeptide receptors presented here and the comparative listing of GPCRs in the hymenopterans S. invicta, Apis mellifera (both eusocial), Nasonia vitripennis (solitary), and the solitary model dipteran Drosophila melanogaster will facilitate comparative endocrinological studies related to social insect evolution and diversity. We compiled the 24 G protein transcripts predicted (15 alpha, 7 beta, and 2 gamma) from 12 G protein genes (5 alpha, 5 beta, and 2 gamma). Reproductive division of labor is extreme in this ant species, therefore, we compared GPCR and G protein gene expression among worker, mated queen and alate virgin queen ant brain transcriptomes. Transcripts for ten GPCRs and two G proteins were differentially expressed between queen and worker brains. The differentially expressed GPCRs are candidate receptors to explore hypotheses on division of labor in this species.