Different time course of changes in sarcoplasmic reticulum and myosin isoforms in rat soleus muscle at early stage of hyperthyroidism.
Different time course of changes in sarcoplasmic reticulum and myosin isoforms in rat soleus muscle at early stage of hyperthyroidism.
复制标题
甲亢早期大鼠比目鱼肌肌浆网和肌球蛋白亚型变化的不同时程[J].
DOI：
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复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
M. Wada
中科院分区：
文献类型：
--
作者：
T. Yamada;S. Inashima;S. Matsunaga;I. Nara;H. Kajihara;M. Wada
目的与方法
AIMS AND METHODS
We studied changes in the expression of myosin and sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA) and in Ca2+ uptake and release rates by the SR in rat slow-twitch soleus muscles treated with thyroid hormone (3,5,3'-triiodo-l-thyronine, T3) for 1, 3, 7 and 21 days.
RESULTS
The content of fast SR Ca2+-ATPase isoform SERCA1a protein significantly increased as early as 3 days of T3 treatment, while a rise in fast myosin heavy chain isoforms was found only 21 days after the onset of T3 administration. T3-induced slow-to-fast transitions were much less pronounced in myosin light chain than in myosin heavy chain. Moreover, in vitro measurements of SR Ca2+-handling capacity with indo-1 indicated that increases in uptake rate preceded those of release rate (7 vs. 21 days).
CONCLUSION
These data suggest that changes in the myosin and SERCA expressions seem not to occur in a coordinated manner at the early stage of hyperthyroidism and that intervention with T3 may temporarily produce slow-twitch type I fibres which contain, in addition to slow SERCA2a isoform, the appreciable amounts of fast SERCA1a isoform. Moreover, it is speculated that some fibres in the hyperthyroid soleus muscles may temporarily acquire unique contractile properties distinct from normal fast and slow fibres.
影响因子：
3.3
作者：
Caiozzo, VJ;Baker, MJ;Baldwin, KM
通讯作者：
Baldwin, KM
DOI：
10.1016/s0021-9258(19)49738-x
发表时间：
1992-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Lytton;M. Westlin;S. E. Burk;G. Shull;D. Maclennan
通讯作者：
J. Lytton;M. Westlin;S. E. Burk;G. Shull;D. Maclennan