Enhanced Optical Manipulation of Cells Using Antireflection Coated Microparticles
Enhanced Optical Manipulation of Cells Using Antireflection Coated Microparticles
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DOI：
10.1021/acsphotonics.5b00178
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发表时间：
2015-10-01
期刊：
影响因子：
7
通讯作者：
Dholakia, Kishan
中科院分区：
文献类型：
--
作者：
Craig, Derek;McDonald, Alison;Dholakia, Kishan
我们证明了使用AR（AR）涂层微粒的增强光学操纵细胞。具体而言，我们孵育CHO-K1，HL 60，和NMuMG细胞系AR涂层的二氧化钛微粒，随后进行阻力测量使用光学捕获。在天然的聚苯乙烯微粒和AR微粒标记的细胞之间进行直接比较。通过在拖曳力实验中测量Q值来记录光阱效率。与AR微粒孵育的CHO-K1细胞显示出与天然细胞相比Q值增加近220%。随着AR微粒的列入，超过50 μ m/s的细胞速度记录只有33 mW的激光捕获功率。使用荧光染料和表达基于荧光遍在蛋白化的细胞周期蛋白（FUCCI）的细胞证实了细胞活力，这证实在AR微粒存在的情况下细胞周期没有受到破坏。
We demonstrate the use of antireflection (AR) coated microparticles for the enhanced optical manipulation of cells. Specifically, we incubate CHO-K1, HL60, and NMuMG cell lines with AR-coated titania micropartides and subsequently performed drag force measurements using optical trapping. Direct comparisons were performed between native, polystyrene microparticle and AR microparticle tagged cells. The optical trapping efficiency was recorded by measuring the Q value in a drag force experiment. CHO-K1 cells incubated with AR microparticles show an increase in the Q value of nearly 220% versus native cells. With the inclusion of AR microparticles, cell velocities exceeding 50 mu m/s were recorded for only 33 mW of laser trapping power. Cell viability was confirmed with fluorescent dyes and cells expressing a fluorescent ubiquitination-based cell cycle protein (FUCCI), which verified no disruption to the cell cycle in the presence of AR microparticles.