Effects of sustained length-dependent activation on in situ cross-bridge dynamics in rat hearts
Effects of sustained length-dependent activation on in situ cross-bridge dynamics in rat hearts
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DOI：
10.1529/biophysj.107.111740
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发表时间：
2007-12-15
影响因子：
3.4
通讯作者：
Yagi, Naoto
中科院分区：
文献类型：
--
作者：
Pearson, James T.;Shirai, Mikiyasu;Yagi, Naoto
在跳动的心脏中，长度依赖性收缩力增加的细胞基础仍不清楚。我们的目的是研究长度依赖性介导的收缩力的增加是否与肌凝蛋白头部接近肌动蛋白有关。悲叹，以及在经济收缩期间被激活的交叉桥的数量。因此，我们采用x射线衍射分析在开胸条件下自发跳动的大鼠心脏的搏动收缩，同时记录左心室（LV）压力-容积。在稳态收缩和急性容量负荷(静脉滴注乳酸林格液60 ml/h，
The cellular basis of the length-dependent increases in contractile force in the beating heart has remained unclear. Our aim was to investigate whether length-dependent mediated increases in contractile force are correlated with myosin head proximity to actin. laments, and presumably the number of cross-bridges activated during a contraction. We therefore employed x-ray diffraction analyses of beat-to-beat contractions in spontaneously beating rat hearts under open-chest conditions simultaneous with recordings of left ventricle (LV) pressure-volume. Regional x-ray diffraction patterns were recorded from the anterior LV free wall under steady-state contractions and during acute volume loading (intravenous lactate Ringers infusion at 60 ml/h,