CHARACTERIZATION OF THE GENE FOR HUMAN NEUTROPHIL-ACTIVATING PEPTIDE-78 (ENA-78)
CHARACTERIZATION OF THE GENE FOR HUMAN NEUTROPHIL-ACTIVATING PEPTIDE-78 (ENA-78)
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DOI：
10.1006/bbrc.1994.2709
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发表时间：
1994-11-30
影响因子：
3.1
通讯作者：
WALZ, A
中科院分区：
文献类型：
--
作者：
CORBETT, MS;SCHMITT, I;WALZ, A
ENA-78的基因组DNA已从人4号染色体流式分选粘粒文库获得。25'000个筛选的单菌落中有3个产生相同的2.2-kB EcoRI ENA-78基因片段。在基因组Southern印迹上观察到类似大小的片段，表明存在单个ENA-78基因。转录起始位点使用5' RACE方案定位于从刺激的肺泡II型上皮细胞（A549）poly（A）mRNA制备的第一链cDNA上。ENA-78基因包含4个外显子和3个内含子，开放阅读框为342个核苷酸，编码114个氨基酸。5'南京区含有几种核因子如AP-2、NF-κ B和干扰素调节因子-1的潜在结合位点。(C)1994年出版社出版。
The genomic DNA for ENA-78 has been obtained from a human chromosome 4 flow-sorted cosmid library. Three out of 25'000 screened single colonies yielded the same 2.2-kB EcoRI ENA-78 gene fragment. A similar size fragment was observed on genomic southern blots, suggesting the presence of a single ENA-78 gene. The transcriptional start site was localized using a 5' RACE protocol on first strand cDNA prepared from stimulated alveolar type-II epithelial cell (A549) poly(A) mRNA. The ENA-78 gene contains four exons and three introns and the open reading frame of 342 nucleotides encodes for a protein of total 114 amino acids. The 5' nanking region contains potential binding sites for several nuclear factors such as AP-2,NF-kappa B, and interferon regulatory factor-1. (C) 1994 Academic Press, Inc.