Expression of Oct4, a stem cell marker, in the hamster pancreatic cancer model
Expression of Oct4, a stem cell marker, in the hamster pancreatic cancer model
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DOI：
10.1159/000094317
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发表时间：
2006-01-01
期刊：
影响因子：
3.6
通讯作者：
Pour, Parviz M.
中科院分区：
文献类型：
--
作者：
Iki, Katsumichi;Pour, Parviz M.
背景：Oct 4已被证明是一种在胚胎细胞和生殖细胞肿瘤中表达的干细胞标志物。近年来，已有报道其在一些人体组织和癌细胞中表达。因为在仓鼠胰腺癌模型中，大多数肿瘤是从胰岛内发展而来的，推测这些胰岛可能来自干细胞，所以我们研究了Oct 4在该模型中的表达。研究方法：用浓度为1：500的单克隆Oct 4抗体对2例正常胰腺和15例由N-亚硝基双（2-氧丙基）胺（BOP）诱导的胰腺癌进行免疫组化处理。结果：正常胰腺组织中，Oct 4仅表达于胰岛细胞，呈弥漫性胞浆表达。在所有细胞中均未发现核染色。在14例胰腺癌中，在许多细胞或小病灶中检测到核染色。1例呈弥漫性胞浆染色，2例呈高尔基体和细胞核混合染色。核染色也被确定在早期岛内导管和钙原位病变。结论：BOP重新激活Oct 4基因，可以被认为是该模型中的早期肿瘤标志物。版权所有（C）2006 S. Karger AG、巴塞尔和IAP。
Background: Oct4 has been shown to present a stem cell marker that is expressed in embryonic cells and in germ cell tumors. Recently, its expression in a few human tissues and cancer cells has been reported. Because in the hamster pancreatic cancer model most tumors develop from within islets presumably from stem cells, we investigated the expression of Oct4 in this model. Methods: Two normal pancreases and 15 pancreatic cancers induced by N-nitrosobis(2-oxypropyl)amine (BOP) were processed for immunohistochemistry using a monoclonal Oct4 antibody at a concentration of 1: 500. Results: In the normal pancreas, Oct4 was expressed only in islet cells in a diffuse cytoplasmic pattern. No nuclear staining was found in any cells. In 14 of the pancreatic cancers, nuclear staining was detected in many cells or in small foci. Diffuse cytoplasmic but no nuclear staining was found in one tumor and a mixed Golgi type and nuclear staining in two cases. Nuclear staining was also identified in early intrainsular ductular and in Ca in situ lesions. Conclusions: BOP reactivates the Oct4 gene and can be considered an early tumor marker in this model. Copyright (C) 2006 S. Karger AG, Basel and IAP.