Congo red effect on cyst viability and cell wall structure of encysting Entamoeba invadens
Congo red effect on cyst viability and cell wall structure of encysting Entamoeba invadens
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DOI：
10.1016/s0188-0128(98)00029-3
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发表时间：
1999-03-01
影响因子：
7.7
通讯作者：
Mora-Galindo, J
中科院分区：
文献类型：
--
作者：
García-Zapién, AG;González-Robles, A;Mora-Galindo, J
背景侵袭内阿米巴包囊的细胞壁是由几丁质微纤维为主要结构成分组成的。已经在酵母中证明，几丁质细胞壁组装被染料如刚果红（CR）和Calcofluoro改变。本工作的目的是研究CR染料对E.在阿米巴经受100- 2，000 μ g CR/mL的作用后，通过光学和电子显微镜观察侵袭虫。实验在BI-S-33和mLG培养基中进行。在BI-S-33培养基中孵育8天后，100- 1，000 μ g/mL CR未抑制滋养体生长。然而，用2，000 μ g/mL染料观察到低水平的生长。当使用100 μ g CR/mL时，24-48 h后形态上可存活（透明）的包囊产量没有显著差异，而最高浓度的CR（2，000 μ g/mL）导致透明包囊产量显著降低;培养物中死亡包囊占主导地位，特别是在CR处理72 h时。在500- 2，000 μ g/mL CR存在下孵育的阿米巴的分化产生异常几丁质沉积物，如透射和扫描电子显微镜所示，呈现不规则的厚细胞壁或双细胞壁。当在BI-S-33中孵育时，在100 μ g/mL CR下获得的包囊培养物产生与对照一样多的滋养体，但在较高CR剂量下获得的培养包囊中仅发现少量滋养体。我们的结果表明，CR影响E。侵入包囊，改变细胞壁的形成，也影响包囊的生存能力。(C)1999年IMSS。出版社：Elsevier Science Inc.
Background. The cell wall of Entamoeba invadens cysts is composed of chitin microfibrils as the main structural component. It has been demonstrated in yeast that the chitin cell wall assembly is altered by dyes such as Congo red (CR) and Calcofluor.Methods. The purpose of this work was to study the cell wall assembly under the effect: of CR dye on encysting E. invadens by means of light and electron microscopy, after the amebas were subjected to the effect of 100-2,000 mu g CR/mL. Experiments were performed either in BI-S-33 or in mLG media.Results. Trophozoite growth was not inhibited by 100-1,000 mu g/mL CR after 8 days of incubation in BI-S-33 medium. However, low levels of growth were observed with 2,000 mu g/mL of dye. No significant differences in morphologically viable (hyaline) cyst production occurred after 24-48 h, when 100 mu g CR/mL was used, while the highest concentration of CR (2,000 mu g/mL) resulted in a significant decrease of hyaline cyst yield; dead cysts prevailed in cultures, particularly at 72 h of CR treatment. Differentiation of amebas incubated in the presence of 500-2,000 mu g/mL CR produced abnormal chitin deposits, rendering irregularly thick or double cell walls, as shown by transmission and scanning electron microscopy. Cyst cultures obtained under 100 mu g/mL CR produced as many trophozoites as did the control when they were incubated in BI-S-33, but only low numbers of trophozoites were found in culture cysts obtained under higher CR doses.Conclusion. Our results suggest that CR affects E. invadens encystment, alters the cell wall formation, and also affects the cyst viability. (C) 1999 IMSS. Published by Elsevier Science Inc.