Investigation of the effect of substrate morphology on MDCK cell mechanical behavior using atomic force microscopy
Investigation of the effect of substrate morphology on MDCK cell mechanical behavior using atomic force microscopy
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DOI：
10.1063/1.5109115
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发表时间：
2019-08
影响因子：
4
通讯作者：
Keyvan Mollaeian;Yi Liu;Siyu Bi;Juan Ren
中科院分区：
文献类型：
--
作者：
Keyvan Mollaeian;Yi Liu;Siyu Bi;Juan Ren
活细胞感知并响应细胞外环境。它们的接触引导受底层衬底形貌的影响。以往关于基质图案对活细胞力学行为影响的研究仅局限于细胞弹性的量化。然而，细胞力学性能的长度和时间尺度如何受到图案基底的影响还有待研究。在这项研究中，利用基于压痕的原子力显微镜，深入研究了衬底形态对活细胞生物力学行为的影响。结果表明，基质形态对细胞生物力学行为有显著影响。在聚二甲基硅氧烷（PDMS）上培养的细胞的弹性和黏度比在扁平PDMS上培养的细胞低得多。细胞的孔弹性扩散系数在有图案的PDMS衬底上较高，特别是在有二维节距的衬底上。此外，荧光图像显示底物形貌直接影响细胞骨架形态。总之，结果表明，细胞的力学行为和形态可以通过适当设计的基底来控制。
Living cells sense and respond to their extracellular environment. Their contact guidance is affected by the underlying substrate morphology. Previous studies of the effect of the substrate pattern on the mechanical behavior of living cells were only limited to the quantification of the cellular elasticity. However, how the length and time scales of the cellular mechanical properties are affected by the patterned substrates are yet to be studied. In this study, the effect of the substrate morphology on the biomechanical behavior of living cells was thoroughly investigated using indentation-based atomic force microscopy. The results showed that the cellular biomechanical behavior was affected by the substrate morphology significantly. The elasticity and viscosity of the cells on the patterned Polydimethylsiloxane (PDMS) substrates were much lower compared to those of the ones cultured on flat PDMS. The poroelastic diffusion coefficient of the cells was higher on the patterned PDMS substrates, specifically on the substrate with 2D pitches. In addition, fluorescence images showed that the substrate topography directly affects the cell cytoskeleton morphology. Together, the results suggested that cell mechanical behavior and morphology can be controlled using substrates with properly designed topography.