Detection of Leptospira spp. in the aqueous humor of horses with naturally acquired recurrent uveitis
Detection of Leptospira spp. in the aqueous humor of horses with naturally acquired recurrent uveitis
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DOI：
10.1128/jcm.38.7.2731-2733.2000
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发表时间：
2000-07-01
影响因子：
9.4
通讯作者：
Willits, NH
中科院分区：
文献类型：
--
作者：
Faber, NA;Crawford, M;Willits, NH
长期以来，人们一直认为钩端螺旋体与马复发性葡萄膜炎有关。本项目旨在确定是否存在钩端螺旋体属。在患有葡萄膜炎的马的房水中，以确定是否与炎症有关。根据临床评价或病史，确定30匹马患有复发性葡萄膜炎。16匹马在临床和历史上被判定为无葡萄膜炎，并用作对照。培养房水样本，并通过PCR评估是否存在钩端螺旋体DNA。收集血清并评价钩端螺旋体病组中5种血清型抗体的存在。通过PCR检测，30匹复发性葡萄膜炎马中的21匹和16匹无葡萄膜炎马中的1匹为钩端螺旋体DNA阳性。在这21匹患有葡萄膜炎的马中，有6匹的房水钩端螺旋体培养呈阳性。血清学结果与房水中钩端螺旋体DNA或微生物的存在没有很好的相关性。钩端螺旋体在自然发生的复发性葡萄膜炎的马中存在很高的百分比。
Leptospiral organisms have long been presumed to be associated with the presence of equine recurrent uveitis. This project was undertaken to determine the presence of Leptospira spp. in the aqueous humor of horses with uveitis to determine if there was an association with inflammation. Thirty horses were determined to have recurrent uveitis based on clinical evaluation or history. Sixteen horses were judged clinically and historically to be free of uveitis and were used as controls. Aqueous humor samples were cultured and evaluated by PCR for the presence of Leptospira DNA. Serum was collected and evaluated for the presence of antibodies against five serovars in a leptospirosis panel, Twenty-one of 30 horses with recurrent uveitis and one of 16 uveitis-free horses were positive by PCR for the presence of Leptospira DNA. Six of these 21 horses with uveitis were culture positive for leptospires from the aqueous humor. Serologic results did not correlate well with the presence of Leptospira DNA or organisms in the aqueous humor. Leptospira spp. are present in a high percentage of horses with naturally occurring recurrent uveitis.