Development of fungiform papillae, taste buds, and their innervation in the hamster.
Development of fungiform papillae, taste buds, and their innervation in the hamster.
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仓鼠菌状乳头、味蕾的发育及其神经支配。
DOI：
10.1002/cne.903400405
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Kachele,DL
中科院分区：
文献类型：
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作者：
Whitehead,MC;Kachele,DL
成熟仓鼠的真菌状味蕾比其他物种的味蕾更少受到神经营养的影响。本研究通过检查生长神经和分化菌状乳头之间的关系来评估仓鼠发育过程中的味蕾神经营养作用。鼓索（CT）或舌（三叉神经）神经（LN）纤维标记荧光黄，因为它们生长到（CT纤维）或周围（LN纤维）发育的味蕾。舌在胚胎期（E）10.5-11天形成，含有位于深部的CT和LN纤维，但没有乳头。到E12时，舌上皮出现散在隆起。这些“隆起”选择性地由LN纤维支配，LN纤维比CT纤维更早地生长到上皮细胞，并且数量更多。E13-14期间，分泌性菌状乳头迅速形成，并受到LN纤维的严重支配。上皮内CT纤维，罕见的E13，总是支配菌状乳头含有新生的味蕾在E14。在E14-15（出生= E15-16），大多数乳头包含味蕾孔，广泛的神经纤维束周围LN神经支配，广泛的神经纤维束内CT神经支配。出生时菌状乳头和味孔的数量与成年时相似，表明菌状隆起开始形成时缺乏神经支配。随后分化的最终菌状乳头和LN纤维的神经支配同步发生，味蕾的分化和CT神经支配之前。仓鼠早熟（例如，与大鼠相比）在LN发育和出生时前舌的结构成熟方面。© Wiley利斯公司
Fungiform taste buds in mature hamsters are less subject to neurotrophic influences than those of other species. This study evaluates taste‐bud neurotrophism during development in hamsters by examining the relation between growing nerves and differentiating fungiform papillae. Chorda tympani (CT) or lingual (trigeminal) nerve (LN) fibers were labelled with Lucifer Yellow as they grew into (CT fibers) or around (LN fibers) developing taste buds. Developing fungiform papillae and taste pores were counted with the aid of a topical tongue stain.The tongue forms on embryonic days (E) 10.5–11 and contains deeply placed CT and LN fibers but no papillae. By E12, the tongue epithelium develops scattered elevations. These “eminences” selectively become innervated by LN fibers that grow to the epithelium earlier and in larger numbers than CT fibers. Definitive fungiform papillae form rapidly during E13–14 and become heavily innervated by LN fibers. Intraepithelial CT fibers, rare at E13, invariably innervate fungiform papillae containing nascent taste buds at E14. During E14–15 (birth = E15–16), most papillae contain taste buds with pores, extensive perigemmal LN innervation, and extensive intragemmal CT innervation. At birth, numbers of fungiform papillae and taste pores are adultlike.The results show that fungiform eminences begin forming in the absence of innervation. The subsequent differentiation of definitive fungiform papillae and their innervation by LN fibers occur synchronously, prior to the differentiation of taste buds and their CT innervation. The hamster is precocious (e.g., compared to rat) in terms of LN development and the structural maturity of the anterior tongue at birth. © Wiley‐Liss, Inc.