High‐affinity Na+/K+‐dependent glutamate transporter EAAT4 is expressed throughout the rat fore‐ and midbrain
High‐affinity Na+/K+‐dependent glutamate transporter EAAT4 is expressed throughout the rat fore‐ and midbrain
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DOI：
10.1002/cne.21823
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发表时间：
2008-11
影响因子：
2.5
通讯作者：
A. Massie;L. Cnops;I. Smolders;R. Mccullumsmith;R. Kooijman;S. Kwak;L. Arckens;Y. Michotte
中科院分区：
文献类型：
--
作者：
A. Massie;L. Cnops;I. Smolders;R. Mccullumsmith;R. Kooijman;S. Kwak;L. Arckens;Y. Michotte
兴奋性氨基酸转运蛋白4(EAAT4)是高亲和力Na+/K+依赖的谷氨酸转运蛋白家族的成员，在小脑浦肯野细胞中高度丰富，但不限于这些细胞。观察EAAT4蛋白在成年大鼠不同脑区的表达情况。尽管与小脑相比，EAAT4蛋白的表达水平中等，但EAAT4蛋白在整个前脑和中脑中普遍存在。用抗小鼠EAAT4 N-末端序列的抗体，在黑质致密部、腹侧被盖区、黑质旁核、缰脚间系统、视上核、丘脑后外侧核、下丘和上丘浅层的蛋白表达水平最高。在海马主细胞、谷氨酸能细胞、γ-氨基丁酸能细胞、多巴胺能细胞和胆碱能细胞以及大脑皮层II/III和V层神经元中也检测到较高水平的EAT4蛋白。原位杂交和逆转录-聚合酶链式反应(RT-PCR)证实EAAT4在一些重要的前脑和中脑结构的mRNA水平上有表达，实时定量聚合酶链式反应估计EAAT4在小脑中的表达范围为6.7~1.6%。J.Comp.神经。511：155-172,2008。©2008 Wiley-Liss，Inc.
Excitatory amino acid transporter 4 (EAAT4), a member of the high‐affinity Na+/K+‐dependent glutamate transporter family, is highly enriched in Purkinje cells of the cerebellum, although it is not restricted to these cells. The detailed expression of EAAT4 protein in different adult rat fore‐ and midbrain regions was examined. Despite moderate expression levels compared with the cerebellum, EAAT4 protein was omnipresent throughout the fore‐ and midbrain. With antibodies raised against the N‐terminal mouse EAAT4 sequence, the highest protein expression levels were observed in the substantia nigra pars compacta, ventral tegmental area, paranigral nucleus, habenulo‐interpeduncular system, supraoptic nucleus, lateral posterior thalamic nucleus, subiculum, and superficial layers of the superior colliculus. Relatively high levels of EAAT4 protein were also detected in the hippocampal principal cells, in the glutamatergic, γ‐aminobutyric acid (GABA)ergic, dopaminergic and most likely cholinergic cells of all nuclei of the basal ganglia, and in neurons of layers II/III and V of the cerebral cortex. The expression of EAAT4 was confirmed at the mRNA level in some important fore‐ and midbrain structures by in situ hybridization and reverse transcriptase‐polymerase chain reaction (RT‐PCR) and estimated to range from 6.7 to 1.6% of the amount in the cerebellum as measured by real‐time PCR. J. Comp. Neurol. 511:155–172, 2008. © 2008 Wiley‐Liss, Inc.