Roles of histone acetylation and chromatin remodeling factor in a meiotic recombination hotspot.
Roles of histone acetylation and chromatin remodeling factor in a meiotic recombination hotspot.
复制标题
组蛋白乙酰化和染色质重塑因子在减数分裂重组热点中的作用。
DOI：
10.1038/sj.emboj.7600138
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Ohta,Kunihiro
中科院分区：
文献类型：
--
作者：
Yamada,Takatomi;Mizuno,Ken-ichi;Hirota,Kouji;Kon,Ning;Wahls,WayneP;Hartsuiker,Edgar;Murofushi,Hiromu;Shibata,Takehiko;Ohta,Kunihiro
组蛋白乙酰转移酶（HAT）和ATP依赖性染色质重塑因子（ADCR）通过调节局部染色质构型参与选择性基因调控。cAMP反应元件（CRE）样序列M26和异源二聚体ATF/CREB转录因子Atf 1·Pcr 1介导的裂殖酵母重组热点6-M26的激活染色质重塑发生在M26周围减数分裂。我们研究了HAT和ADCR在M26周围染色质重塑中的作用。在减数分裂早期，M26周围的组蛋白H3和H4以M26和Atf 1依赖的方式过度乙酰化。SpGcn5，S. Gcn 5 p的pombehomolog是体内M26周围组蛋白H3乙酰化的主要条件。gcn 5+的缺失导致了染色质重塑的显著延迟，但仅部分降低了M26减数分裂重组频率。这些结果表明，HAT和ADCR协同改变局部染色质结构，如选择性转录激活，以位点特异的方式激活M26的减数分裂重组。
Histone acetyltransferases (HATs) and ATP‐dependent chromatin remodeling factors (ADCRs) are involved in selective gene regulation via modulation of local chromatin configuration. Activation of the recombination hotspotade6‐M26ofSchizosaccharomyces pombeis mediated by a cAMP responsive element (CRE)‐like sequence,M26, and a heterodimeric ATF/CREB transcription factor, Atf1·Pcr1. Chromatin remodeling occurs meiotically aroundM26. We examined the roles of HATs and ADCRs in chromatin remodeling aroundM26. Histones H3 and H4 aroundM26were hyperacetylated in anM26‐ and Atf1‐dependent manner early in meiosis. SpGcn5, theS. pombehomolog of Gcn5p, was required for the majority of histone H3 acetylation aroundM26 in vivo. Deletion ofgcn5+caused a significant delay in chromatin remodeling but only partial reduction ofM26meiotic recombination frequency. Thesnf22+(a Swi2/Snf2‐ADCR homologue) deletion andsnf22+gcn5+double deletion abolished chromatin remodeling and significant reduction of meiotic recombination aroundM26.These results suggest that HATs and ADCRs cooperatively alter local chromatin structure, as in selective transcription activation, to activate meiotic recombination atM26in a site‐specific manner.