Establishment of Aedes (Ochlerotatus) scapularis (Diptera: Culicidae) in Mainland Florida, With Notes on the Ochlerotatus Group in the United States
Establishment of Aedes (Ochlerotatus) scapularis (Diptera: Culicidae) in Mainland Florida, With Notes on the Ochlerotatus Group in the United States
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DOI：
10.1093/jme/tjaa250
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发表时间：
2021-03-01
影响因子：
2.1
通讯作者：
Burkett-Cadena, Nathan D.
中科院分区：
文献类型：
--
作者：
Reeves, Lawrence E.;Medina, Johana;Burkett-Cadena, Nathan D.
肩胛斑伊蚊（Aedes scapularis）（Rondani）是一种分布广泛的新热带病媒蚊种，根据1945年在佛罗里达中部群岛收集的三个幼虫标本，已被列入佛罗里达的蚊子区系。在这里，我们报告了许多最近收集的未成熟和成年的AE。来自佛罗里达南部两个县多个地点的肩胛骨。这些标本代表了Ae的第一个记录。来自佛罗里达大陆的肩胛骨，也是自75年前首次发现该物种以来，该州首次记录到该物种。几年来收集的幼虫和成虫表明，Ae。现在布劳沃德县和迈阿密戴德县建立了肩胛肌。这些当代记录本物种在佛罗里达可能代表新的传播和随后建立的事件，从美国以外的人口或最近重新出现的未被发现的地方性种群。目的：进一步明确山慈菇的形态学鉴定。对来自佛罗里达的肩胛斑伊蚊标本的细胞色素c氧化酶亚基I基因（COI）的DNA条形码区域进行测序，并与来自美国的所有其他黄斑伊蚊组物种（特别是Aedes condolescens Dyar和Knab）进行比较（双翅目：蚊科）、Aedes infirmatus Dyar and Knab（双翅目：蚊科）、白纹伊蚊（双翅目：蚊科）、扭纹伊蚊（Theobald）（双翅目：蚊科）和三纹伊蚊（Coquillett）（双翅目：蚊科）。分子检测和测序证实了Ae的形态鉴定。肩胛肌标本COI和ITS2序列的最大似然系统发育分析将佛罗里达Ae. scapularis在一个不同的分支，但无法产生明显的分支为佛罗里达标本的Ae。condolescens和Ae.折磨人[图形]。
Aedes scapularis (Rondani), a widespread neotropical vector mosquito species, has been included in the mosquito fauna of Florida on the basis of just three larval specimens that were collected in the middle Florida Keys in 1945. Here, we report numerous recent collections of immature and adult Ae. scapularis from multiple locations in two counties of southern Florida. These specimens represent the first records of Ae. scapularis from mainland Florida and the first records of the species in the state since the initial detection of the species 75 yr ago. Collections of both larvae and adults across several years indicate that Ae. scapularis is now established in Broward and Miami-Dade Counties. These contemporary records of this species in Florida may represent novel dispersal and subsequent establishment events from populations outside the United States or a recent reemergence of undetected endemic populations. To confirm morphological identification of Ae. scapularis specimens from Florida, the DNA barcoding region of the cytochrome c oxidase subunit I gene (COI) was sequenced and compared to all other Ochlerotatus Group species from the United States, specifically Aedes condolescens Dyar and Knab (Diptera: Culicidae), Aedes infirmatus Dyar and Knab (Diptera: Culicidae), Aedes thelcter Dyar (Diptera: Culicidae), Aedes tortilis (Theobald) (Diptera: Culicidae), and Aedes trivittatus (Coquillett) (Diptera: Culicidae). Molecular assays and sequencing confirm morphological identification of Ae. scapularis specimens. Maximum likelihood phylogenetic analysis of COI and ITS2 sequences place Florida Ae. scapularis in a distinct clade, but was unable to produce distinct clades for Florida specimens of Ae. condolescens and Ae. tortilis.[GRAPHICS].