A high pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry ion source for thermal stabilization of labile biomolecules.
A high pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry ion source for thermal stabilization of labile biomolecules.
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高压基质辅助激光解吸/电离傅里叶变换质谱离子源，用于不稳定生物分子的热稳定。
DOI：
10.1002/rcm.447
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Costello,CE
中科院分区：
文献类型：
--
作者：
O'Connor,PB;Costello,CE
设计并测试了高压基质辅助激光解吸/电离（MALDI）傅里叶变换质谱（FTMS）离子源。通过这种设计，在解吸期间，在MALDI样品的区域中，压力被脉冲至估计的1-10毫巴，结果与在<0.1毫巴的压力下操作的类似系统相比，碎裂显著减少。 在这些条件下，振动激发离子的热稳定性显示为从“热”基质α-氰基-4-羟基肉桂酸解吸的小肽，以及胰岛素的高度不稳定的氧化β链。具有几个唾液酸残基的脆性神经节苷脂在高压下解吸，并且保持完整而没有唾液酸的典型损失，并且蛋白质标准品泛素（8565.64 Da）以最小的脱水解吸。 在高压碰撞冷却条件下，分子离子的非共价基质加合物变得突出，但在FT质谱仪中捕获离子的情况下，离子可以被轻度激活以分离基质加合物。此外，新的来源产生大量的多电荷离子，这在MALDI-TOFMS中很常见，但在MALDI-FTMS中很少观察到。这种效应更可能是由于消除了先前FTMS离子源中的质量过滤效应，而不是离子的碰撞冷却。版权所有© 2001约翰威利父子有限公司。
A high pressure matrix‐assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry (FTMS) ion source was designed and tested. With this design, pressure is pulsed to an estimated 1–10 mbar in the region of the MALDI sample during desorption with the result of significantly decreased fragmentation compared to similar systems operating with pressures of <0.1 mbar. The thermal stabilization of vibrationally excited ions under these conditions is shown with small peptides desorbed from the ‘hot’ matrix α‐cyano‐4‐hydroxycinnamic acid, and with the highly labile oxidized β‐chain of insulin. Fragile gangliosides with several sialic acid residues are desorbed under high pressure and remain intact without the typical losses of sialic acid, and a protein standard, ubiquitin (8565.64 Da), is desorbed with minimal dehydration. Under high pressure collisional cooling conditions, non‐covalent matrix adduction to the molecular ions becomes prominent, but with the trapped ions in an FT mass spectrometer, the ions can be mildly activated to detach the matrix adducts. The new source, additionally, generates significant levels of the multiply charged ions which are commonly seen in MALDI‐TOFMS, but are rarely observed in MALDI‐FTMS. This effect is more likely due to the elimination of a mass filtering effect in the previous FTMS ion source than to collisional cooling of the ions. Copyright © 2001 John Wiley & Sons, Ltd.