A biomaterial approach to cell reprogramming and differentiation.
A biomaterial approach to cell reprogramming and differentiation.
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DOI：
10.1039/c6tb03130g
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发表时间：
2017-04-07
期刊：
影响因子：
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通讯作者：
Kim DH
中科院分区：
文献类型：
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作者：
Long J;Kim H;Kim D;Lee JB;Kim DH
体细胞重新编程为多能状态，随后分化为某些表型，有助于促进再生医学研究和其他医学应用。最近的研究已经使用病毒载体来诱导这种重新编程；然而，限制包括低效率和安全问题。在这篇综述中，我们讨论了生物材料方法如何为提高病毒方法的生存能力和下游适用性提供潜在的途径，或者提供一种更安全的替代方法。非病毒传递系统的使用，如电穿孔、微/纳米颗粒、核酸以及对培养底物形貌和硬度的调节，产生了关于细胞重新编程的有价值的见解。改进细胞重编程方法对再生医学的努力至关重要。这篇综述讨论了利用生物材料方法重新编程的进展。
Cell reprogramming of somatic cells into pluripotent states and subsequent differentiation into certain phenotypes has helped progress regenerative medicine research and other medical applications. Recent research has used viral vectors to induce this reprogramming; however, limitations include low efficiency and safety concerns. In this review, we discuss how biomaterial methods offer potential avenues for either increasing viability and downstream applicability of viral methods, or providing a safer alternative. The use of non-viral delivery systems, such as electroporation, micro/nanoparticles, nucleic acids and the modulation of culture substrate topography and stiffness have generated valuable insights regarding cell reprogramming. Improving cell reprogramming methods is vital to regenerative medicine efforts. This review discusses advances in reprogramming using biomaterial approaches.