Expression of connexin 43 mRNA in microisolated murine osteoclasts and regulation of bone resorption in vitro by gap junction inhibitors
Expression of connexin 43 mRNA in microisolated murine osteoclasts and regulation of bone resorption in vitro by gap junction inhibitors
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DOI：
10.1016/s0006-291x(03)00502-3
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发表时间：
2003-04-18
影响因子：
3.1
通讯作者：
Lie, A
中科院分区：
文献类型：
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作者：
Ransjö, M;Sahli, J;Lie, A
多项研究表明，连接蛋白43 （Cx43）介导对成骨细胞功能和成骨重要的信号。间隙连接通讯在骨吸收中的作用尚不清楚。我们研究了Cx43 mRNA在破骨细胞和骨吸收培养物中的表达，并进一步研究了骨吸收中间隙连接通讯的功能重要性。RT-PCR分析显示Cx43 mRNA在小鼠骨髓培养物和骨髓培养物微分离的破骨细胞中表达。Cx43 mRNA在骨吸收培养物中也有表达，这些骨吸收培养物与破骨细胞和成骨细胞/雌激素细胞在失活骨片上孵育48小时。在甲状旁腺（PTH）刺激的（0.1 nM）骨吸收中检测到Cx43 mRNA的上调。18 α -甘草次酸（30 muM）和油酰胺（100 muM）这两种间隙连接通讯抑制剂显著抑制PTH-和1,25-(OH)(2) d -3刺激的破骨细胞窝形成。总之，我们的数据表明了骨吸收中缝隙连接通讯的功能作用。(C) 2003 Elsevier Science（美国）版权所有。
Several studies have demonstrated that connexin 43 (Cx43) mediates signals important for osteoblast function and osteogenesis. The role of gap junctional communication in bone resorption is less clear. We have investigated the expression of Cx43 mRNA in osteoclasts and bone resorption cultures and furthermore, the functional importance of gap junctional communication in bone resorption. RT-PCR analysis demonstrated Cx43 mRNA expression in mouse bone marrow cultures and in osteoclasts microisolated from the marrow cultures. Cx43 mRNA was also expressed in bone resorption cultures with osteoclasts and osteoblasts/stronial cells incubated for 48 h on devitalized bone slices. An up-regulation of Cx43 mRNA was detected in parathyroid (PTH)-stimulated (0.1 nM) bone resorption. Two inhibitors of gap junction communication, 18alpha-glycyrrhetinic acid (30 muM) and oleamide (100 muM), significantly inhibited PTH- and 1,25-(OH)(2)D-3-stimulated osteoclastic pit formation. In conclusion, our data indicate a functional role for gap junction communication in bone resorption. (C) 2003 Elsevier Science (USA). All rights reserved.