The atheroprotective roles of heart-protecting musk pills against atherosclerosis development in apolipoprotein E-deficient mice
The atheroprotective roles of heart-protecting musk pills against atherosclerosis development in apolipoprotein E-deficient mice
复制标题
麝香护心丸对载脂蛋白 E 缺陷小鼠动脉粥样硬化的预防作用
DOI：
10.21037/atm.2019.12.22
复制
发表时间：
2019-12-01
影响因子：
--
通讯作者：
Guo, Xiaomei
中科院分区：
文献类型：
--
作者：
Lu, Li;Qin, Yating;Guo, Xiaomei
背景资料：麝香保心丸（HMP）是一种具有抗动脉粥样硬化相关性心血管疾病（CVD）作用的中药制剂，其抗动脉粥样硬化的作用机制尚不清楚。在这里，我们研究了HMP对减轻小鼠动脉粥样硬化病变程度的影响，并探讨其分子机制。方法：载脂蛋白E缺陷小鼠喂饲西式饲料补充HMP（25 mg/kg/d）或生理盐水灌胃20周。然后进行组织病理学染色以评估动脉粥样硬化斑块负荷。生化试剂盒用于检测血脂水平。此外，与肝脏和肠道组织中的脂质代谢的效应因子进行了研究，通过蛋白质印迹和实时荧光定量PCR分析。结果：HMP能显著缩小动脉粥样硬化斑块的大小（P
Background: Heart-protecting musk pill (HMP), derived from Chinese herbal medicines, has been found to possess protective roles against atherosclerosis-related cardiovascular diseases (CVDs), however, the anti-atherosclerotic mechanisms of HMP are still unclear. Here, we investigated the effects of HMP on alleviating atherosclerotic lesion severity in mice and explored the molecular mechanisms.Methods: Apolipoprotein E-deficient mice were fed western-type diet supplemented with HMP (25 mg/kg/day) or normal saline gavage for 20 weeks. Then histopathological staining was performed to assess the atheromatous plaque burden. Biochemical kits were used to detect levels of lipid profiles. Moreover, effector factors associated with lipid metabolism in liver and intestinal tissues were investigated by western blot and real-time PCR assays. Levels of signal molecules participating in the mitochondrial-mediated apoptosis pathway were detected by Western blot.Results: We found that HMP notably reduced atherosclerotic lesion size (P